复方斯亚旦生发油的HPLC指纹图谱研究

Study on HPLC Fingerprint of Fufang Siyadan Oil

目的建立复方斯亚旦生发油的高效液相色谱(HPLC)指纹图谱,为评价该制剂的整体质量提供依据。方法采用中药色谱指纹图谱相似度评价系统(2012年版)对复方斯亚旦生发油的HPLC指纹图谱进行相似度评价,并使用SPSS 19.0软件进行聚类分析。结果选取了8个色谱峰作为指纹图谱共有峰,10批样品的相似度计算结果均>0.95;通过聚类分析可将10批样品聚为4类。结论建立的复方斯亚旦生发油HPLC指纹图谱方法稳定可行。

OBJECTIVE To establish high performance liquid chromatography(HPLC) fingerprints of Fufang Siyadan Oil to provide a reference for evaluation of the overall quality of this preparation. METHODS The similarity of the HPLC fingerprint of Fufang Siyadan Oil analyzed with 'Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica 2012A', and hierarchical clustering analysis(HCA) was performed by SPSS 19.0. RESULTS There were 8 common peaks, and the similarity degrees of 10 batches of samples were >0.95, and showed that all the samples were of good consistency. The samples were divided into 4 clusters by HCA. CONCLUSION Establishment of fingerprint can provide more comprehensive reference for the quality control of Fufang Siyadan Oil. The established HPLC fingerprint method is stable and feasible.