三桠苦枝叶化学成分的分离、鉴定

Isolation and Identification of Chemical Constituents from Twigs and Leaves of Evodia lepta(Spreng.) Merr.

目的研究三桠苦[Evodia lepta (Spreng.) Merr.]枝叶的化学成分,为三桠苦的进一步研究奠定物质基础。方法对三桠苦无水乙醇提取物的乙酸乙酯萃取物采用硅胶柱层析分离纯化,得到单体化合物,经波谱分析鉴定其化合物结构。结果从三桠苦乙酸乙酯萃取物中分离得到6个单体化合物,分别为异吴茱萸酮酚(化合物1)、异吴茱萸酮酚甲醚(化合物2)、3,5-二羟基-4-乙氧基-6-乙酰基-7-甲氧基-2,2-二甲基苯并二氢吡喃(化合物3)、(cis)-3,4,5-三羟基-6-乙酰基-7-甲氧基-2,2-二甲基色烷(化合物4)和(trans)-3,4-二羟基-5-甲氧基-6-乙酰基-7-甲氧基-2,2-二甲基色烷(化合物5)和对羟基苯甲酸丁酯(化合物6)。结论化合物1～6均为首次从三桠苦植物的枝叶中分离得到。

OBJECTIVE To study the chemical constituents from the twigs and leaves of Evodia lepta(Spreng.) Merr.. In order to lay a substantial foundation for the further research. METHODS The ethyl acetate fraction of anhydrous ethanol extract from Evodia lepta(Spreng.) Merr. was separated and purified by silica gel column chromatography. The structures of the compounds were identified by nuclear magnetic resonance(NMR) spectrometry. RESULTS Six compounds were isolated as isoevodionol(compound 1), isoevodionol methyl ether(compound 2), 3,5-dihydroxy-4-ethoxy-6-acetyl-7-methoxy-2,2-dimethylchroman(compound 3),(cis)-3,4,5-trihydroxy-6-acetyl-7-methoxy-2,2-dimethylchroman(compound 4),(trans)-3,4-dihydroxy-5-methoxy-6-acetyl-7-methoxy-2,2-dimethylchroman(compound 5), butylparaben(compound 6). CONCLUSION The compounds(compound 1-6) are isolated from the twigs and leaves of Evodia lepta(Spreng.) Merr. for the first time.