败酱草单萜环烯醚酯类对Hep G2、MCF7细胞增殖及凋亡的影响?

Effects of PMIEE on Proliferation and Apoptosis of HepG2 and MCF7 cells

目的探讨败酱草单萜环烯醚酯类(patrinia monoterpene iridoid ether esters,PMIEE)对HepG2和MCF7细胞增殖抑制和凋亡的影响。方法 HepG2和MCF7细胞经PMIEE作用后,采用CCK8法检测细胞增殖抑制情况;Annexin V-FITC/PI双标记流式细胞术检测细胞凋亡及周期情况;细胞划痕实验检测细胞迁移状况;Western blot法检测Bcl-2、Bax、caspase3、cdc2和CyclinB1的表达情况。结果 CCK8、划痕实验和Annexin V-FITC/PI流式细胞术检测显示,PMIEE对HepG2和MCF7细胞均有显著的增殖抑制、促凋亡率和降低迁移率作用(P<0.05),呈一定量效关系,且PMIEE对HepG2细胞的周期阻滞以G2/M期为主,MCF7细胞以G0/G1期为主;Western blot结果显示,PMIEE可显著下调2种细胞Bcl-2、cdc2、CyclinB1的表达,上调Bax和caspase3的表达水平。结论 PMIEE可诱导HepG2和MCF7细胞增殖抑制和凋亡,下调Bcl-2、cdc2和CyclinB1表达及上调Bax和caspase3表达,其抗癌的潜在机制可能与此有关。

OBJECTIVE To investigate the effects of Patrinia monoterpene iridoid ether esters(PMIEE) on the proliferation and apoptosis of HepG2 and MCF7 cells. METHODS The HepG2 and MCF7 cells were cultered in vitro. CCK8 assay was used to detect cell proliferation inhibition and Annexin V-FITC/PI double labeling flow cytometry was used to detect cell apoptosis and cell cycle, and cell scratch assay was used to detect cell migration. Detection of the expression of Bcl-2, Bax,caspase-3, cdc2 and CyclinB1 by Western blot. RESULTS CCK8, scratch and Annexin V-FITC/PI flow cytometry showed that PMIEE significantly inhibited the proliferation of HepG2 and MCF7 cells, and promoted apoptosis and decreased mobility(P<0.05), showed a dose-effect relationship. The cycle arrest of HepG2 cells by PMIEE was mainly in G2/M phase, while MCF7 cells was mainly in G0/G phase. The results of Western blot showed that PMIEE could significantly down-regulate the expression of Bcl-2, cdc2, CyclinB1 and up-regulate the expression of Bax and caspase3 in two kinds of cells. CONCLUSION PMIEE can induce proliferation inhibition and apoptosis of HepG2 and MCF7 cells, down-regulate the expression of Bcl-2, cdc2 and CyclinB1, and up-regulate the expression of Bax and caspase3. While its potential related with anti-cancer mechanism.