金蝉花水提物对高糖诱导的肾小管上皮细胞转分化的影响

Effects of Cicada Aqueous Extract on the Transdifferentiation of Renal Tubular Epithelial Cells Induced with High Glucose

目的研究金蝉花水提物对高糖诱导下肾小管上皮细胞(HK-2)发生上皮-间充质细胞转分化的影响。方法采用高糖诱导HK-2细胞建立糖尿病肾病模型,将细胞分为正常组,高糖组,贝那普利组,金蝉花水提物低、高剂量组(0.1,0.5 mg·L^(-1)),培养24 h后,ELISA测定转化生长因子β1(transforming growth factor-?1,TGF-β1)、纤维连接蛋白(fibronectin,FN),Western blot测定α-平滑肌肌动蛋白(?-smooth muscle actin,α-SMA)的表达情况。结果与正常组比,高糖组TGF-β1、α-SMA和FN蛋白表达显著增高(P<0.01);与高糖组比,贝那普利组和金蝉花水提物高剂量组TGF-β1、α-SMA和FN蛋白表达显著降低(P<0.01)。结论金蝉花水提物可能通过降低TGF-β1、α-SMA和FN蛋白的表达,从而缓解甚至逆转肾小球的硬化与肾间质细胞纤维化,发挥治疗糖尿病肾病的作用。

OBJECTIVE To study the effect of cicada aqueous extract on epithelial-mesenchymal transition of renal tubular epithelial cells(HK-2)induced by high glucose.METHODS HK-2 cells induced by high glucose were used to establish diabetic nephropathy model.The cells were divided into normal group,high glucose group,benazepril group,and cicada aqueous extract low and high dose group(0.1,0.5 mg·L-1).After 24 hours of culture,the expression of TGF-β1 and FN were determined by ELISA,α-SMA was determined by Western blot.RESULTS Compared with the normal group,the expression ofα-SMA,TGF-β1 and FN protein in the high glucose group was significantly increased(P<0.01).Compared with high glucose group,the indexs in benazepril group and cicada aqueous extract high dose group were significantly decreased(P<0.01).CONCLUSION Cicada aqueous extract may reduce the expression of TGF-β1,α-SMA and FN proteins,so as to relieve or even reverse glomerular sclerosis and renal interstitial cell fibrosis and achieve the effect of treating diabetic nephropathy.