UPLC-MS/MS测定安神类中成药和保健食品中8种非法添加抗抑郁类化学成分

Determination of Eight Antidepressants Illegally Added In Tranquilizing Traditional Chinese Medicine and Health Food by UPLC-MS/MS

目的建立了安神类中成药和保健食品中的8种非法添加抗抑郁类化学成分的超高效液相色谱-三重四级杆串联质谱(UPLC-MS/MS)检测方法。方法以甲醇为提取溶剂,Waters ACQUITY UPLC BEH C_(18)(2.1 mm×100 mm,1.7μm)色谱柱分离,三重四级杆质谱仪检测,MRM模式定性、定量检测安神类中成药和保健食品中的8种抗抑郁类化学成分(文拉法辛、西酞普兰、帕罗西汀、阿米替林、氟西汀、三甲丙咪嗪、舍曲林和氯丙咪嗪)。结果 8种抗抑郁类化学成分质谱检测的线性范围宽,相关性好,r≥0.9955;方法精密度RSD为1.5%～4.6%;方法回收率为88.2%～103.2%;定量限为0.02～0.03μg·L^(-1);日内精密度的RSD(n=5)为1.1%～3.8%,日间精密度的RSD(n=9)为1.4%～3.7%。结论本方法专属性强,操作简单,快捷,可作为安神类中成药和保健食品中8种抗抑郁类化学成分定量、定性的有效检测方法。

OBJECTIVE To develop a ultra performance liquid chromatography coupled with triple quadrupole mass spectrometry(UPLC-MS/MS)method to determine 8 antidepressants in tranquilizing traditional Chinese medicine and health food.METHODS The analysis was performed by a UPLC-MS/MS system with Waters ACQUITY UPLC BEH C18(2.1 mm×100 mm,1.7μm)column.Multiple-reaction monitoring(MRM)was performed to identify and quantify 8 antidepressants(venlafaxine,citalopram,paroxetine,amitriptyline,trimepramine,fluoxetine,sertraline and clomipramine),which were extracted from samples with methanol.RESULTS Linear calibration curves were obtained with r≥0.995 5.The precision of the method was showed by RSDs(n=6)ranging from 1.5%to 4.6%.The recoveries were determined at three concentration levels and ranged from 88.2%to 103.2%.The ranges of LOQs were from 0.02 to 0.03μg·L-1 and the RSDs(n=9)of intra-day precision and inter-day precision were from 1.1%to 3.8%and from 1.4%to 3.7%,respectively.CONCLUSION The method is specific,simple and rapid to detect 8 antidepressants in tranquilizing traditional Chinese medicine and health food.