泰山紫草与新疆紫草的HPLC指纹图谱对比研究

Comparison of HPLC Fingerprints Between Lithospermum Erythrorhizon and Arnebia Euchroma

目的对比研究泰山紫草和新疆紫草指纹图谱及其主要成分含量,评价泰山紫草的药用价值。方法采用HPLC,色谱柱为Symmetry C_(18_色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-水(70∶30),流速为1.0 mL·min^(-1),柱温为30℃,检测波长为516nm。应用中药色谱指纹图谱相似度评价系统(2012年版)软件,建立泰山紫草、新疆紫草指纹图谱。结果建立了泰山紫草、新疆紫草指纹图谱的共有模式,以新疆紫草对照图谱作为参照图谱,进行峰匹配,对比计算泰山紫草指纹图谱的相似度为0.798,泰山紫草中含有萘醌类有效成分,但含量与新疆紫草中存在差异。结论泰山紫草具有一定的药用价值,但不可完全替代新疆紫草使用。

OBJECTIVE To compare the fingerprints and contents of the main components of Lithospermum erythrorhizon and Arnebia euchroma,and to evaluate the medicinal value of Lithospermum erythrorhizon.METHODS HPLC was used as followed:the chromatographic column of Symmetry C18 column(4.6 mm×250 mm,5μm)was used with the mobile phase of acetonitrile-water(70∶30),the flow rate was 1.0 mL·min-1,the column temperature was 30℃,and the detection wavelength was set at 516 nm.The fingerprints of Lithospermum erythrorhizon and Arnebia euchroma were established by using the software of'Similarity Evaluation System of Chromatographic Fingerprints of Traditional Chinese Medicine(2012 edition)'.RESULTS A common pattern of the fingerprints of Lithospermum erythrorhizon and Arnebia euchroma was established.The control map of Arnebia euchroma was used as reference map for peak matching.The similarity of the fingerprints of Lithospermum erythrorhizon and Arnebia euchroma was calculated to be 0.798.Lithospermum erythrorhizon contained naphthraquinone,but its content was different from that of Arnebia euchroma.CONCLUSION Lithospermum erythrorhizon has certain medicinal value,but it can not completely replace the Arnebia euchroma.