中药饮片紫苏子和钩藤的耐热菌考察

Investigation of Thermoduric Bacteria in Chinese Herbal Medicine: Uncaria Rhynchophylla and Perilla Frutescens

目的考察中药饮片紫苏子和钩藤中耐热菌数随加热时间增加的变化情况,为相关标准制定提供数据支持。方法按照中国药典2020年版中药饮片微生物限度检查法(增订草案)中的方法对2种饮片进行计数方法适用性检查,然后测定不同沸水浴时间耐热菌数,分离鉴定存活的耐热菌,并进行灭活实验。结果检验结果符合中国药典2020版通则1107(修订草案)。随着沸水浴时间的延长,耐热菌数量显著下降。鉴定结果显示耐热菌株均为芽孢杆菌属。结论中药饮片存在一定的耐热菌污染,应建立合理的耐热菌质控标准,保障中药饮片的用药安全。

OBJECTIVE To investigate the change in thermoduric bacteria count of Uncaria rhynchophylla and Perilla frutescens as heating time increased,in order to provide data support for the establishment of related standards.METHODS According to the microbial limit test method for Chinese herbal medicine of Chinese Pharmacopoeia 2020 edition(draft amendment),the suitability of the counting method of these two herbal medicine was examined.Then the amount of thermoduric bacteria were determined at different boiling bath time.The surviving thermoduric bacteria were isolated and identified for an inactivation test.RESULTS The results were in compliance with 1107 general chapter of Chinese Pharmacopoeia 2020 edition(draft amendment).The counts of thermoduric bacteria were significantly reduced as boiling bath time increased.The identification results indicated that all the thermoduric bacteria belong to the Bacillus.CONCLUSION Chinese herbal medicines are contaminated by thermoduric bacteria,a reasonable thermoduric bacteria control standards shall be established to ensure the safety of medication.