微量热法研究壳聚糖铜对核桃内生菌生长代谢的影响

Microcalorimetric Study on Interaction of Chitosan Copper and Endophyte of Walnut in Walnuton

使用加热回流方式制备了配合物壳聚糖铜(CTS-Cu),采用红外光谱法和原子吸收光谱法对配合物进行了表征,结果表明CTS中的-OH与-NH_2可能参与了配位。采用原子吸收光谱法测定CTS-Cu溶液中Cu^(2+)的含量,同时利用等温微量热方法研究了CTS-Cu、Cu^(2+)和CTS分别对核桃内生菌(XRS107)生长代谢的影响,测定结果表明CTS-Cu参与了对XRS107的生长代谢过程,并对XRS107的生长代谢过程的影响表现出"低浓度刺激,高浓度抑制"的特征,而Cu^(2+)和CTS对XRS107的生长代谢过程无明显影响。光密度法研究结果表明,经不同浓度CTS-Cu作用后,XRS107的光密度值(OD)表现出先增大后减小的趋势。

In this paper ,CTS‐Cu was prepared by heating reflux ,and the complex was analyzed by FT‐IR and AAS .The results showed‐OH and‐NH2 could be involved in the coordination .The content of Cu2+ in complex was calculated by AAS . Furthermore , the metabolism of walnut endophytes (XRS107) with CTS‐Cu ,Cu2+ and CTS were detected by isothermal microcalorimetric method respec‐tively .The results showed that the complex CTS‐Cu was involved in the metabolic process of XRS107 .In the CTS‐Cu concentration range ,the effect on the growth of XRS107 showed the charac‐teristics of Hormesisi effect ,while Cu2+ and CTS had no obvious influence on XRS107 growth metab‐olism .In addition ,the values of optical density (OD) of XRS107 was evaluated with low concentra‐tions of CTS‐Cu ,and was dropped with high concentrations of CTS‐Cu .