亚硒酸钠诱导麦苗GSH-Px生物合成机理的探讨

STUDIES ON THE MECHANISM OF SODIUM SELENITE-INDUCED GLUTATHIONE PEROXIDASE BIOSYNTHESIS OF WHEAT SEEDLINGS

试验表明:1.0mg·L-1的Na2SeO3可以提高麦苗地上部GSH Px的活性。试验选用抑制效果相似的转录抑制剂AMD和翻译抑制剂CHI,分别或共同与1.0mg·L-1的Na2SeO3配合处理麦苗,都能抑制由Na2SeO3诱导的GSH Px的活性,72h后其抑制效果相似,差异不显著;而不和Na2SeO3配合的处理,差异却十分显著。试验表明:Na2SeO3对GSH Px的合成的诱导除在转录或翻译水平上外,还存在其他途径。

Treatment of wheat seedlings with Na_2SeO_3 at 1.0 mg/L increased the activity of GSH-Px (glutathione peroxidase) . Na_2SeO_3 (1.0 mg/L) combined with the transcription inhibitor Actinomycin D (AMD) or the translation inhibitor Cyclonheximede (CHI) or both inhibited Na_2SeO_3-induced GSH-Px activity with no significant difference between them as determined 72 h after the treatments. Significant difference was observed between the two inhibitors in their inhibitory effect when they were used alone, without combination with Na_2SeO_3. The experiment showed that Na_2SeO_3-induced GSH-Px biosynthesis happens before translation, not after it and that other pathway of induction may exist addition to the known pathways on the transcription and translation levels.