^(60)Co辐照诱变对姬松茸菌丝生长及其细胞形态结构的影响

EFFECT OF^(60)Co γ-RAYS ON GROWTH OF Agaricus blazei MURILL HYPHA AND ITS CELL SHAPE STRUCTURE

本文报道了经不同剂量60 Coγ射线辐照对姬松茸菌丝生长、扭结和细胞形态结构的影响。结果表明 ,采用 0 2～ 0 5kGy低剂量辐射后的姬松茸菌丝 ,细胞壁比对照厚 ,而且细胞出现重度质壁分离。随着辐照剂量增大其细胞壁则变薄 ,而且细胞出现轻度质壁分离。经高剂量辐射后 ,姬松茸菌丝生长速度减缓 ,菌丝生长呈稀弱状 ,但与对照相比 ,采用低剂量辐射则有利于促进菌丝生长和提前扭结 ,其子实体增产率达 34 8%。

The effect of 60Co γ-rays at different dosage on growth, tangle and structure of cell shape of Agarius blazei Murill(Abm) hypha were studied. The experiment results showed that the cell wall of treatment Abm hypha was thicker than that of the control, huge separation between cytoplasm and cell wall of Abm hypha appeared when Abm hypha was irradiated at the low dosage (0.2～0.5kGy). The cell wall became thin and small separation between cytoplasm and cell wall appeared with the increase of the irradiation dosage. The growth of Abm hypha became slow and scare by irradiation with higher dosage. The growth and tangle of hypha could be improved and the yield of fruit body increased by 34.8% when irradiated with low dosage.