婚检人群乙、丙型肝炎、梅毒及艾滋病毒检测

Determination on HBV,HCV,syphilis and HIV infection in population of health checkup before marriage

目的 了解北京市婚检人群乙型和丙型肝炎、梅毒及艾滋病病毒感染情况。方法 乙型肝炎表面抗原、丙型肝炎抗体和艾滋病毒抗体采用酶免法检测 ;梅毒采用快速血浆反应素环状卡片试验 (RPR)和梅毒螺旋体血凝试验 (TPHA)检测 :乙型和丙型肝炎病毒载量采用PCR荧光定量方法检测 ,病毒基因型采用直接PCR基因分型法测定。结果 80 0例婚检人群乙型和丙型肝炎感染率分别为 12 0 % ,6 0 % ,梅毒和艾滋病病毒未检出。 96例乙肝表面抗原阳性婚检人群中 ,乙型肝炎病毒载量小于 10 5 拷贝 /ml者占 88 5% ,大于 10 5 拷贝 /ml者仅占 11 5%。 5例丙型肝炎病毒RNA阳性者中 ,病毒载量均小于 10 4 拷贝 /ml。该婚检人群HBV感染者中 ,B型占 18 6% ,B和C混合型占42 4% ,C型占 2 3 7%。 3例丙型肝炎病毒感染者均为HCV1b型。结论 该婚检人群中 ,乙型和丙型肝炎病毒感染率相对较高 ,但病毒载量相对较低 。

ObjectiveTo study HBV,HCV,syphilis and HIV infections in the population of health checkup before marriage in Beijing. MethodsHBsAg,anti-HCV and anti-HIV were detected by enzy me-linked immunosorbent assays (ELISAs),syphilis was tested by a rapid plasma re gain assay (RPR) and a Treponema pallidum hemagglutination assay (TPHA).Viral lo ad of HBV and HCV were measured by fluorescence quantitative polymerase chain re actions (FQ-PCR),and HBV and HCV genotypes were determined by PCR using type-spe cific primers. ResultsIn 800 individuals of health checkup before marri age in Beijing,the prevalence ra tes of HBV,HCV infections were 12 0% and 6 0%,respectively.No syphilis and HIV infections were detected.In 96 HBsAg positive individuals,88.5% had the HBV loa ds less than 10\+5 copies/ml ,and 11 5% had more than 10 5 copies/ml.18 6% were infected with HBV B genotype alone,42 4% coinfected with B and C genotype s,and 23 7% with genotype C alone.3 HCV individuals were all infected with geno type 1b. ConclusionThe HBV and HCV prevalence rates were relative ly higher,and no syphilis and HIV infections were detected in the population of health checkup before marriage in Beijing.