链脲佐菌素对大鼠肝微粒体LPO模型的影响

Effect of streptozocin on LPO in rat microsomal models

目的 研究链脲佐菌素对大鼠肝微粒体LPO模型的影响及其剂量效应关系。方法 以钙沉淀法提取二级雄性SD大鼠肝微粒体 ,分别建立CCl4 、CHP和Vc/Fe2 + 等激发的微粒体LPO反应模型。向三类反应模型中加入不同剂量的链脲佐菌素 ,并调节至终浓度 :0 3 ,0 6,0 9,1 2 ,1 5,1 8mg/ml,分别测定LPO反应产物MDA的生成量。结果 与对照组相比 ,各实验组MDA产量均显著增加 (P <0 .0 1) ,并且随着链脲佐菌素剂量的增高 ,MDA产量亦增加 ,呈现良好的剂量效应关系。在三类模型中MDA产量随不同剂量的链脲佐菌素变化趋势相一致。结论 链脲佐菌素能加重大鼠肝微粒体LPO模型的脂质过氧化损伤 ,并且具有剂量

ObjectiveTo investigate the effect of streptozocin on LP O in rat microsomal model and its dose-effect relationship. MethodsHepatic microsome was extracted from clean male S D rats by calcium ion precipitating.Three LPO models stimulated by CCl 4,CHP an d Vc/Fe 2+ were built respectively.Streptozocin was added into three models and regulated to final concentration: 0.3,0.6,0.9,1.2,1.5,1.8?mg/ml.MDA,a LPO product,was determined after the reaction. ResultsCompared with control model,the MDA in all trial groups was significantly increased(P<0.01).With streptozocin increasing in trial groups,MDA was increased correspondingly,which showed a linear dose-effect relationship between streptozocin and MDA.There was a similar dose-effect relat ionship in three models.ConclusionStreptozocin can cause oxidative damage of mic rosome and increase MDA product.There was a dose-effect relationship between str eptozocin and MDA.