食管鳞癌中CD151、ERK1/2及血管生成拟态的表达及临床意义

Expression of CD151 and ERK1/2 in Esophageal Squamous Cell Carcinoma and Their Correlation with Vasculogenic Mimicry

[目的]研究组织分化抗原151(CD151)及细胞外调节蛋白激酶1/2(ERK1/2)在食管鳞状细胞癌(ESCC)中的表达情况,分析两者表达与血管生成拟态(VM)的相关性。[方法]选取100例存档石蜡包埋ESCC组织标本作为观察组和50例癌旁(≥5cm)正常食管组织作为对照组,采用免疫组织化学ElivisionTMplus法检测ESCC中CD151及ERK1/2的表达,运用CD34/PAS双染检测食管癌中VM存在情况;并用Kaplan-Meier法分析VM与预后的关系。[结果 ]观察组CD151(82%)、ERK1/2(75%)蛋白阳性表达率及VM存在率(29%)均明显高于对照组(10%、36%、8%)(P均<0.05)。CD151蛋白表达与ESCC的浸润深度、淋巴结转移、临床分期、分化程度有关(P均<0.05);ERK1/2蛋白表达与ESCC的淋巴结转移、临床分期、分化程度有关(P均<0.05);ERK1/2在CD151阳性组的表达明显高于CD151阴性组,且两者表达呈正相关(r=0.451,P<0.001);CD151和ERK1/2表达均与VM存在呈正相关(r=0.299,P=0.002;r=0.318,P=0.001)。Kaplan-Meier生存分析显示有VM组与无VM组5年生存率分别为10.3%和50.7%(P<0.05)。Cox多因素回归分析显示:浸润深度、临床分期、VM存在是食管鳞癌患者术后5年生存率的独立影响因素(P均<0.05)。[结论] CD151、ERK1/2在食管鳞癌的发生及发展中具有重要作用,可能通过VM的形成,影响食管鳞癌的发生发展、转移及预后。

[Objective]To investigate the expression of tissue differentiation antigen 151(CD151)and extracellular regulated protein kinase1/2(ERK1/2)in esophageal squamous cell carcinoma(ESCC),and to analyze the their correlation with vasculogenic mimicry(VM).[Methods]One hundred samples of paraffin-embedded ESCC tissue and 50 samples of normal esophageal tissues were collected.The expression of CD151 and ERK1/2 in ESCC was detected by ElivisionTM plus method.The VM in esophageal cancer was detected by CD34/PAS double staining.The relationship between VM and prognosis was analyzed by Kaplan-Meier method.[Results]The positive rate of CD151(82%),ERK1/2(75%)and the present rate of VM(29%)in the ESCC group were significantly higher than those in the control group(10%,36%,8%;all P<0.05).The expression of CD151 protein was correlated with the depth of invasion,lymph node metastasis,clinical stage and degree of differentiation of ESCC(all P<0.05).The expression of ERK1/2 protein was correlated with lymph node metastasis,clinical stage and differentiation of ESCC(all P<0.05).The expression of ERK1/2 was positively correlated with the expression of CD151 in ESCC(r=0.451,P<0.001);the expression of CD151 and ERK1/2 was positively correlated with VM(r=0.299,P=0.002;r=0.318,P=0.001).Kaplan-Meier survival curve showed that the 5-years survival rate of VM group and non-VM group was 10.3%and 50.7%,respectively(P<0.05).Cox multivariate regression analysis demonstrated that the depth of invasion,clinical stage and VM were the independent factors of the 5-years survival(P<0.05).[Conclusion]The expression of CD151 and ERK1/2 are upregulated in ESCC,which may affect the development,metastasis and prognosis of ESCC through VM formation.