ENO1在胰腺上皮内瘤变和胰腺导管腺癌中的表达

Expression of ENO1 in Pancreatic Intraepithelial Neoplasia and Pancreatic Ductal Adenocarcinoma

[目的]检测烯醇化酶-1(ENO1)在人和大鼠胰腺上皮内瘤变(PanIN)和胰腺导管腺癌组织中的表达情况。[方法]收集病理科存档人组织标本,胰腺导管腺癌18例,PanIN 4例,正常胰腺组织7例,免疫组化检测ENO1蛋白表达。SD大鼠20只采用胰腺包埋种植7,12-二甲基苯并蒽(DMBA)诱导法,建立大鼠PanIN和胰腺导管腺癌模型,Real-time PCR检测ENO1 mRNA表达,免疫组化和免疫印迹检测ENO1蛋白表达。[结果 ] ENO1蛋白在人PanIN(9.63±1.80)和胰腺导管腺癌组织(12.14±2.71)中的表达均显著高于人正常胰腺组织(P<0.01)。与造模后大鼠正常胰腺比较,ENO1 mRNA在大鼠PanIN-3(P<0.01)和胰腺导管腺癌组织(P<0.001)中表达显著增加,在大鼠PanIN-2(P<0.001)、PanIN-3(P<0.01)和PDAC(P<0.001)组织中,ENO1蛋白表达呈进行性升高。[结论] ENO1在PanIN和胰腺导管腺癌的表达显著增高,在胰腺上皮的癌变过程中可能发挥重要作用。

[Objective]To investigate the expression of enolase 1(ENO1)in pancreatic intraepithelial neoplasia(PanIN)and pancreatic ductal adenocarcinoma(PDAC)tissues from human and rats.[Methods]Eighteen tissue samples of PDAC,4 samples of PanIN and 7 samples of human normal pancreas tissue were collected,the expression of ENO1 protein was detected by immunohistochemistry.The rat model of PanIN and PDAC was induced by implantation with 7,12-dimethylbenzanthracene(DMBA)into pancreas in 20 adult male SD rats.The expression levels of ENO1 mRNA and protein in rat PanIN and PDAC tissues were detected by real-time PCR,immunohistochemistry and Western-blot,respectively.[Results]The expression of ENO1 protein in human PanIN(P<0.01)and PDAC(P<0.001)tissues was significantly higher than that in normal human pancreatic tissues.The expression of ENO1 mRNA was significantly increased in rat DMBA-induced PanIN-3(P<0.01)and PDAC(P<0.001),and the expression of ENO1 protein was also significantly increased in rats PanIN-2(P<0.001),PanIN-3(P<0.01)and PDAC tissues(P<0.001)compared to normal rat pancreas.[Conclusion]ENO1 expression is upregulated in PanIN and PDAC,indicating that ENO1 may be involved in the process of pancreatic epithelial carcinogenesis.