离子对RP-HPLC测定盐酸丁螺环酮缓释片中主药的含量

Determination of the buspirone hydrochloride contents in Buspirone hydrochloride sustained release tablets by ion-pair RP-HPLC

目的 测定盐酸丁螺环酮缓释片中盐酸丁螺环酮的含量。方法 采用离子对反相高效液相色谱法。Nova PakC18色谱柱 ,流动相为甲醇 -乙腈 -离子溶液 (十二烷基硫酸钠 2 5g ,二乙胺 1ml,加水至 10 0 0ml,冰醋酸调pH 3 0 ) (10∶5 0∶4 0 ) ,检测波长为 2 6 5nm。结果 盐酸丁螺环酮的含量在 0 32～ 1 6 μg·ml-1浓度范围内线性关系良好 (r =0 9999)。平均回收率 97 5 6 %(n =5 )RSD =1.13%。结论 所用方法简便、快速、准确。

OBJECTIVE To determine the buspirone hydrochloride contents in Buspirone hydrochloride sustained-release tablets.METHODS Ion-pair RP-HPLC was used with Nova-Pak C 18 column.The mobile phase was methanol-acetonitrile-ionic solution (containing 2.5 g sodium laury1 sulphate and 1 ml diethylamine,added water to 1000 ml and adjusting to pH 3.0 with glacial acetic acid) (10∶50∶40).The UV detective wavelength was at 265 nm.RESULTS There was a good linear relationship within the concentration range of 0.32-1.6 μg·ml -1 of buspirone hydrochloride(r=0.9999).The average recovery was 97.56% with RSD of 1.13%(n=5).CONCLUSION This method is simple,fast and accurate.