不同类型多不饱和脂肪酸对产蛋鸡免疫功能及肝脏脂质过氧化的影响

EFFECTS OF DIFFERENT TYPES OF POLY UNSAT URATED FATTY ACIDS ON HUMORAL IMMUNE FUN CTION AND HEPATIC LIPID PEROXIDATION OF LAYING HENS

目的 : 研究不同类型多不饱和脂肪酸 (PUFA)对产蛋鸡体液免疫功能、淋巴细胞前列腺素 E2 合成及机体脂质过氧化的影响。方法 : 360只 60 w龄的海兰褐产蛋鸡随机分为 1 0个处理组 ,各组试验日粮为 :对照组 (不添加油脂 ) ;鱼油三个添加水平 (1 %、3%、5% ) ;胡麻油三个添加水平 (2 %、4%、6% ) ;玉米油三个添加水平 (2 %、4%、6% )。结果 : 添加不饱和油脂均可以提高血清抗体效价 ,其中鱼油和胡麻油添加组与对照组相比差异显著 (P<0 .0 5) ;添加玉米油组的血清溶菌酶含量显著低于其它油脂添加组和对照组 (P<0 .0 1 ) ;添加鱼油和胡麻油组的外周血淋巴细胞 PGE2 的合成量显著低于添加玉米油组 (P<0 .0 5) ;不同水平多不饱和脂肪酸的添加影响了肝脏组织脂质过氧化分解产物丙二醛 (MDA)的含量 ,随着不饱和油脂添加量的提高 ,MDA含量呈上升的趋势 ,但是 6%玉米油组显著低于鱼油 5%添加组 (P<0 .0 5)。结论 : 在本试验条件下 。

Objective: Effect of different types of polyunsaturated fatty acids on humoral immune function, PGE 2 synthesis by lymphocytes and hepatic lipid peroxidation was investigated in laying hens during an experimental period of 10 weeks. Methods: Three hundred and sixty Hylan laying hens aged 60 weeks were randomly allocated into ten groups and fed corn and soybean meal-based diets added different source and level of fat : control diet (no additional fat), 3 fish oil inclusion levels (FO;1%,3%,5%), 3 linseed oil inclusion levels (LO;2%,4%,6%) or 3 corn oil inclusion levels (CO;2%,4%,6%). Results: Results showed that anti-bovine serum albumin (BSA) antibody titers in hens fed fish oil or linseed oil were higher (P<0.05) compared with that in hens fed the control diet. There were no significant differences (P>0.05) in laying performance hens between different diet groups. The serum lysozyme activity of hens fed corn oil was lower than other groups (P<0.01). The PGE 2 production of peripheral blood leukocytes (PBL) in FO or LO-fed hens are lower (P<0.05) than CO-fed hens. Hens fed high dose oils had more thiobarbituric reactive substances in liver than the control (P<0.05).Conclusion: Different types of PUFA could affect the hepatic lipid peroxidation and immune competence of laying hens.