摘要
目的 : 研究 4种大麦麦叶中提取的黄酮类化合物对超氧阴离子自由基 (O2· )、羟自由基 (· OH)的清除作用。方法 : 用氮蓝四唑 (NBT)光还原法测定 O2·的清除率 ,氧化还原法测定· OH的清除率。结果 : 随着黄酮在反应液中浓度的增加 ,清除率呈上升趋势 ,当黄酮浓度达到 1 2μg/ ml左右时 ,对 O2·和· OH的清除率可分别高达 95.56%和 94.1 2 %。结论 :体外实验证实 。
Objective: To study the scavenging capacity of extract from four kinds of barley leaves on superoxide free radical and hydroxyl free radical. Methods: Measure the content of superoxide free radical and hydroxyl free radical by nitro blue tetrazolium(NBT) photo-reduction. Results: Along with the flavonoid in the reaction liquid, the scavenging rate shows ascending trend . When the concentration of flavonoid is 12μg/ml, its scavenging rate is 95.56% and 94.12% on superoxide free radical and hydroxyl free radical respectively. Conclusion: The flavonoids of barley leave s have stronger anti-oxidative action.
出处
《营养学报》
CAS
CSCD
北大核心
2003年第4期401-404,共4页
Acta Nutrimenta Sinica
关键词
大麦麦叶
黄酮类化合物
自由基
动态研究
barley leaves
flavonoid
scavenge
superoxide free radical
hydroxyl free radical
