新疆厚皮甜瓜抗白粉病基因SSR分子标记

SSR Markers Linked to Powdery Mildew Resistance Gene in Cucumis melo ssp. melo of Xinjiang

【目的】研究厚皮甜瓜抗源的抗白粉病遗传规律,寻找和定位与厚皮甜瓜抗白粉病基因连锁的分子标记,为厚皮甜瓜抗白粉病病基因的定位、克隆和抗病育种等奠定基础。【方法】在明确新疆地区瓜类白粉病生理小种的基础上,以抗病资源收集一号和感病农家品种皇后、F1、BC1P1、BC1P2、F2群体为材料。苗期接种鉴定,分析抗源收集一号抗性遗传规律,并利用SSR标记进行遗传连锁分析。【结果】收集一号对白粉病Podosphaera xanthii生理小种1的抗性由显性单基因控制,对143对SSR引物进行筛选,引物P173-174扩增出的特异性片段与抗病基因表现连锁关系,该片段大小为140 bp,与抗病基因遗传连锁距离为24.0 cM。【结论】P173-174可作为甜瓜抗白粉病分子育种的备选分子标记。

【Objective】The SSR makers linked to powdery mildew resistance gene in muskmelon were studied as they are useful for new resistance genes and the rational usage of marker- assisted selection in muskmelon resistance breeding.【Method】Podosphaera xanthii race 1 caused melon( Cucumis meloL.) powdery mildew was the prevailing race in Xinjiang. Parental lines and( No. ISouji × Huanghou) F 1,F 2 and backcross populations were scored for response to inoculation with P. xanthii race 1. The simple sequence repeat( SSR) technique was used to identify molecular markers linked to the resistance of melon powdery mildew. 【Result】The resistance to P. xanthii race 1 in Souji 1 was controlled by one single dominant gene. A total of 143 pairs SSR primers could detect the polymorphism between the resistant pool and susceptible pool. A unique 140 bp fragment was amplified with the primer P 173- 174. It was identified that it was linked to resistance gene of Souji 1 at a distance of 24. 0 CM. 【Conclusion】This SSR bands indicated that the marker could be useful for marker assisted selection in melon breeding for powdery mildew resistance.