海岛棉丛生芽的诱导及再生

Induction and Regeneration of Clustered Buds from Sea-island Cotton

【目的】探索最佳海岛棉丛生芽诱导及再生的激素组合,为海岛棉的遗传转化奠定基础。【方法】以海岛棉品种新海13号、新海14号和新海16号的茎尖为材料,研究不同激素浓度和激素配比对海岛棉茎尖丛生芽诱导和生根的影响。【结果】在MSB培养基中添加1.0 mg/L的6-BA时,丛生芽生长状况最好,平均丛生芽率最高,为41%;在1/2 MSB培养基中添加浓度为0.3 mg/L的NAA和3.0 mg/L的IBA,平均生根率达到60%。【结论】不同品种海岛棉丛生芽的诱导存在一定差异,但均可获得再生植株。

【Objective】The project aims to explore the best island cotton buds induction and regeneration hormone combination,which might lay foundations for the genetic transformation of Gossypium barbadense Linn.【Method】In this study,stem tips of xinhai 13,xinhai 14 and xinhai 16 were used as the material to explore the influence of different hormones and their proportion to the caespitose shoots' induction and root regeneration. 【Result】The results indicated that stem tips had the best growth status and the highest caespitose shoots induction rate( 41%) in the MS medium containing 1. 0 mg / L 6- BA; The average rate of root regeneration could be 60% in the 1 /2MS medium with additional 0. 3 mg / L NAA and 3. 0 mg / L IBA. 【Conclusion】Regeneration plants can all be acquired though the differences among different varieties exist.