中国美利奴(新疆型)羊毛弯曲频率性状的全基因组关联分析

Genome-wide Association Studies on the Wool Staple Crimp Frequency in Chinese Merino Sheep(Xinjiang Type)

【目的】鉴别影响细羊毛弯曲频率性状的基因组区域。【方法】利用Ovine SNP50 Bead Chip芯片对235只中国美利奴(新疆型)个体基因分型,基于Case/Control设计对弯曲频率性状进行全基因组关联分析。【结果】通过基因组水平的Permutations校正,检测到18个与细羊毛弯曲频率性状显著关联的SNPs。5个SNPs定位于已知基因内(内含子),13个SNPs分别邻近已知基因(距离1.8 kb^841.39 kb)。多数候选基因/SNP均为首次检测到与羊毛性状相关,其中基因PML、LAMC2、PDGF、CDC42SE2及DiRas3参与了人、小鼠毛发生长、发育相关的生物学过程。【结论】鉴别出了与羊毛弯曲频率性状关联的新的候选基因/SNP,对这些目标区域的进一步研究有助于揭示细毛羊羊毛弯曲性状的遗传机理。

【Objective 】 To identify genomic regions and genes that play roles in wool staple crimp.【Method】The genome- wide association studies( GWAS) were performed on staple crimp frequency based on Case / Control method using genotypes from the Ovine SNP50 Bead Chip. The data consisted of 365 animals from the Chinese Merino sheep( Xinjiang Type). 【Result】After adjusting for multiple testing based on permutations,18 genome- wise significant SNPs were identified for staple crimp frequency. Five detected SNPs were located within known genes,and 13 SNPs were located close to the known genes( 1. 8kb- 841. 39 kb apart). The majority of candidate genes were not reported previously to have association with wool traits. The five genes of PML,LAMC2,PDGF,CDC42SE2 and DiRas3 were highlighted,considering their functions of involving in the different signaling pathway related to hair development and growth. 【Conclusion】New candidate regions for wool staple crimp frequency were detected here,and future studies targeting these areas could provide further knowledge to uncover the genetic architecture underlying staple crimp traits in Chinese Merino sheep.