脑缺血再灌注损伤过程中脑的病理变化与脑内脑源性神经营养因子的基因表达

Cerebral ischemia and reperfusion pathological changes and regional brain-derived neurotrophic factor mRNA level expression

目的 研究在脑缺血 -再灌注损伤过程中 ,脑组织的病理变化与脑源性神经营养因子 (brain derivedneurotrophic factor,BDNF)基因表达之间的关系。方法 采用 Zea longa线栓法创建局部脑缺血 -再灌注模型。光镜下观察病理变化 ,原位寡核酸分子杂交检测在脑缺血 -再灌注损伤过程中 ,脑的不同区域 BDNF m RNA的表达 ,图像分析 BDNF的间接定量水平。结果 (1)脑缺血和脑缺血再灌注均可导致 BDNF m RNA在脑的广泛区域表达活跃 ;(2 )损伤过重反而抑制 BDNF m RNA的表达 ;(3 ) BDNF m RNA的表达具有组织差异性 ;(4)脑缺血 -再灌注后 ,早期 BDNF m RNA的表达水平即明显增加 ,12 h达高峰。结论 BDNF的提高是一种保护性反应 ,脑缺血和脑缺血再灌注损伤均可以导致 BDNF m RNA在脑的广泛区域表达水平明显提高 ,表达水平与局部神经元的抗损伤能力呈正相关 。

Objective To study the relationship between the change of b rain and regional brain-derived neurotrophic factor after the cerebral ischemic reperfusion.Methods To use Zea longa for establishing a model of cerebral ischemic reperfusion in the part of the brain in which the level of regional BDNF mRNA (of different parts of the brain) could be measured by using in situ hybridization.Results (1)The high level of BDNF mRNA wa s found in the wide-ranging brain after the cerebral ischemia and the reperfusi on.(2)The lower level of BDNFmRNA in the serious ischemia group was found than t hat in the slight.(3)BDNF mRNA expression was different in various tissues.(4)BDNF mRNA expression increased to the maximum at 12h after the reperfusion.Conclu sion (1)The increase of BDNF plays an neuroprotective role after the r eperfusion.(2)The high level of BDNF mRNA can be found in the wide-ranging brai n after the cerebral ischemia and the reperfusion.(3)The expression of BDNF may be inhibited by the serious ischemia.