胶束电动毛细管色谱法测定复方酮康唑霜中酮康唑和特美呋的含量

Determination of ketoconazole and clobetasol propionate contents in its preparation with micellar electrokinetic chromatography

目的:毛细管电泳法测定酮康唑霜中酮康唑和特美味的含量。方法:分离缓冲液为胆汁酸钠(70 mmol/L)-三羟甲基氨基甲烷-磷酸(50 mmol/L)(pH 8.14),分离电压30 kV、温度25 C、50 μm(内径)×48.5cm(有效长度40cm)空心熔融石英毛细管柱、检测波长240nm。结果:以丙酸睾酮为内标,酮康唑质量浓度在140.5～702.5 μg/ml、特美呋质量浓度在151～755μg/ml之间成良好的线性关系;加样回收率分别为95.4％～103.9％、95.9％～102.6％,RSD％分别为2.8％～4.2％(n=3)、2.9％～3.6％。结论:本法简便、快速、可靠,可用于该复方制剂中酮康唑及特美呋的含量测定。

Objective:To determine ketoconazole and clobetasol propionate contents in its preparation with capillary elec-trophoresis. Methods: The buffer solution containing sodium cholate(70 mmol/L)-Tris-phosphorate(50 mmol/L)(pH 8. 14) was used. The effective length of 50 μm(i. d) fused silica capillary was 40 cm and the detection wavelength was 240 nm. The running voltage was 20 kV and temperature was 25 C. Results:Using testosterone propionate as the internal control, good linear relationships were acquired when ketoconazole concentration ranging 140. 5-702. 5 fig/ml and clobetasol propionate concentration ranging 151-755 μg/ml respectively. The recovery were 95. 4%-103. 9%, 95. 9%-102. 6% with RSD% being 2. 8%-4. 2%(n = 3) and 2. 9%-3. 6% .respectively. Conclusion: This method is simple,fast and reliable .which can be used to determine ketoconazole and clobetasol propionate in its preparations.