卫益颗粒对流感病毒FM_1株感染小鼠血清中细胞因子水平的影响

Effect of weiyi Granules to the level of serous cytokine of mice Infected by FM1 strain virus

目的 :观察卫益颗粒对病毒性肺炎小鼠血清中细胞因子水平的影响。方法 :用流感病毒鼠肺适应株FM1感染的小鼠肺炎模型 ,应用胸腺细胞法测其血清中IL - 1,用结晶紫染色法测其TNF -α对L92 9细胞的杀伤作用 ,应用淋巴母细胞法测其IL - 2。结果 :模型组小鼠较正常对照组血清中IL - 1及TNF -α水平显著升高 (P <0 .0 5 ) ,IL - 2水平显著降低 (P <0 .0 5 ) ,用卫益颗粒治疗后 ,血清中IL - 1、TNF -α水平显著降低 (P <0 .0 5 ,0 .0 1) ,IL - 2水平明显升高 (P <0 .0 1)。结论 :卫益颗粒通过抑制IL -1、TNF -α的过量产生而减轻病毒感染引起的免疫病理损害 ,并通过促进IL - 2的产生 ,提高机体抗病毒感染的细胞免疫功能。

This study is to observe the effect of Weiyi Granules to serous cytokine of mice with viral pneuonia.The mouse viral pneumonia model was created by the infection of FM1 strain virus.We use different method to measure the level of IL-1 IL-2 and TNF-a of mice infected by FM1 strains Virus.The results show Weiyi Granules can reduce immune pathological damage by control excessive produce of TNF-α and IL-1,and improve the function of cell-mediated immune response by raising the produce of IL-2.It can conclude that Weiyi Granules may have the function of resisting influenza virus. Author's address:Beijing University of Traditional Chinese Medicine,Beijing 100029,China