金银花不同部位化学成分的比较研究

A Comparison of Chemical Constituents in Different Parts of Honeysuckle

为了比较金银花茎、叶和花中的化学成分含量,采用了硫酸-蒽酮法、苯酚-硫酸法、2,6-二氯酚靛酚滴定法、考马斯亮蓝法、茚三酮呈色法和紫外分光光度法,分别测定了金银花茎、叶和花中的黄酮类物质、维生素C、绿原酸、可溶性蛋白质、游离氨基酸、可溶性糖和多糖的含量。结果表明:黄酮类化合物和维生素C含量均为叶>花>茎;而绿原酸、可溶性蛋白质和游离氨基酸含量均为:花>叶>茎,虽然花中含量最高,但叶和茎中也有一定的含量;可溶性糖:茎>花>叶;多糖含量:茎>叶>花。这为金银花的综合利用提供了一定的理论依据。

This study aims to compare the chemical constituents in stems,leaves and flowers of honeysuckle.The contents of flavonoids,vitamin C,chlorogenic acid,soluble protein,free amino acid,soluble sugar and polysaccharide are measured by sulfuric acid-anthrone method,phenol-sulfuric acid method,2,6-di chlorphenol indophenol titration method,Coomassie brilliant blue method,ninhydrin coloration method,and ultraviolet spectrophotometry.Results showed that the content of flavonoids and vitamin C in honeysuckle organs were leaf>flower>stem,the content of chlorogenic acid,soluble protein and free amino acid in honeysuckle organs were flower>leaf>stem,although the content in flower was the highest,there were also certain contents in leaves and stems;the soluble sugar content in honeysuckle organs was stem>flower>leaf,and the polysaccharide content was stem>leaf>flower.This study provides a basis for comprehensive utilization of honeysuckle.