白介素-6及血清肿瘤坏死因子-α诊断尖锐湿疣的检验分析

The value of serum tumor necrosis factor alpha and interleukin -6 in the diagnosis of condyloma acuminatum

目的探讨血清肿瘤坏死因子-α(TNF-α)及白介素-6(IL-6)诊断尖锐湿疣的价值。方法选取2015年6月至2017年3月鄂东医疗集团黄石市中心医院(湖北理工学院附属医院)诊治的81例尖锐湿疣患者作为研究对象,将这81例患者设为观察组。另选取2015年6月至2017年3月鄂东医疗集团黄石市中心医院(湖北理工学院附属医院)接待的56例健康体检者作为对照组。再将观察组81例患者中的尖锐湿疣初发患者30例设为初发组,尖锐湿疣复发患者51例设为复发组。入组研究对象均于清晨空腹抽取3 mL外周静脉血,分离血清,采用酶联免疫吸附法(ELISA法)测定血清TNF-α和IL-6含量。比较观察组和对照组组间血清TNF-α和IL-6含量变化,观察组组内所分的初发组和复发组血清TNF-α和IL-6含量变化,及TNF-α+IL-6诊断方法学效能。结果观察组血清TNF-α和IL-6含量低于对照组,且差异具有统计学意义(P<0.05);初发组血清TNF-α和IL-6含量高于对照组,且差异具有统计学意义(P<0.05);TNF-α+IL-6诊断特异度和灵敏度高于TNF-α和IL-6单项检测。结论尖锐湿疣患者血清TNF-α和IL-6含量均降低,TNF-α+IL-6诊断特异度和灵敏度较高,具有重要临床研究价值。

Objective To investigate the value of serum tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in the diagnosis of condylomaacuminatum(CA).Methods 81 patients with CA and 56 healthy people for physical examination in Huangshi Central Hospital of Eastern Hubei Medical Group from June 2015 to March 2017 were selected as the observation group and control group respectively.Of the 81 patients in the observation group,30 ones with initial CA were regarded as the initial group and the rest 51 ones with recurrent CA as the recurrent group.3 mL fasting peripheral venous blood was taken and the levels of serum TNF-αand IL-6 were measured by enzyme-linked immunosorbent assay(ELISA).The changes in serum TNF-αand IL-6 levels were compared between the observation group and the control group,as well as between the initial group and the recurrent groups.The diagnostic efficacy of TNF-αand IL-6 was also compared.Results The levels of serum TNF-αand IL-6 in the observation group were lower than those in control group,with statistically significant differences(P<0 05).The levels of serum TNF-αand IL-6 in the initial group were significantly higher than those in the control group,with statistically significant differences(P<0 05).The specificity and sensitivity of TNF-αjoint with IL-6 was higher than those of TNF-αand IL-6 alone.Conclusions The serum levels of TNF-αand IL-6 in patients with CA are lower with high diagnostic specificity and sensitivity,which is of important clinical value.