小鼠胚胎发育中期心脏细胞增殖与凋亡的研究

Cell Proliferation Apoptosis and the Expression of Related Genes in the Developing Heart of Mouse

采用免疫组织化学及切口末端标记法(TUNEL),对胚胎中期的小鼠心脏组织进行检测,结果表明:小鼠心脏发育过程中的细胞增殖与凋亡相伴存在,与心脏发育变化密切相关.而用原位杂交的方法检测,P21、Ki ras基因在胚胎心脏发育中期并没有表达,说明这2个细胞周期调控基因在胚胎心脏发育中期并不起作用,可能与其他的基因有关.

Immunohistochemistry and TUNEL techniques were adopted to observe the variation of cell proliferation and apoptosis and the expression characteristics of P 21 and Ki-ras. We discovered that cell proliferation was accompanied by apoptosis during the development of mouse heart. The dynamic changes of both ratios might be correlated to the different structures appearing in different developmental process. The possible function of P 21 and Ki-ras might be not correlated to the developmental process of middle embryo heart.