天然植物制剂AMH体外抑制多种强致癌物的致突变作用

The inhibitory effect of the natural plant extract AMH on the mutagenicity of several carcinogens in vitro

目的 研究开发具有防癌、抗突变的生物资源 ,为预防致癌物致突变物对遗传物质的损害、预防肿瘤和生物资源的开发利用提供科学依据。方法 改进的鼠伤寒沙门氏菌 /哺乳动物肝微粒体酶试验 (Ames试验 )。结果 由云南一特色真菌植物提取的天然植物制剂 (AMH) ,经试验研究证明 ,对生产生活中多种常见的强致癌物、致突变物如 :2 氨基芴 (2 AF)、苯并 (a)芘(BaP)、黄曲霉毒素 (AFB1)、环磷酰胺 (CP)、卷烟焦油 (CSC)、染发剂 (HCC)、二甲基亚硝胺 (DMN)、二乙基亚硝胺 (DEN)等的致突变作用具有很强的抑制作用 (抑制率达 80 %～ 10 0 % ,P <0 .0 5 ) ,并具有很好的剂量反应关系。AMH为水溶性 ,富含有机硒和黄酮类物质 ,稳定性好 ,经毒理学安全性评价安全无毒。AMH在卷烟、化装品及日常生产生活的防癌抗突变方面具有广阔的应用前景。结论 AMH对所检测的 8种生产生活中的强致癌物、致突变物的致突变作用具有显著的抑制作用 ,在利用生物资源防癌抗突变方面具有很好的应用前景。

Objective To observe the antimutagenic effect of anti-mutagenic herb (AMH) on 2-aminofluorene (2-AF), bezo(a)pyrene (B(a)P),afflatoxin B 1(AFB 1), cyclophosphamide(CP), cigarette smoke condensate (CSC), hair coloring cream (HCC), dimethylnitrosamine (DMN) and diethylnitrosamine (DEN). Method Salmonella typhimurium/mammals microsomal enzyme test (Ames test). Results The AMH could markedly inhibit the mutagenicity of those carcinogens and mutagens common in human living environment, the inhibitory rates were 80%-100%. A good dose-effect relationships were presented. Conclusion The study proves that AMH is able to significantly inhibit the mutagenicities of the tested 8 carcinogens and mutagens.