增生性瘢痕内应激活化蛋白激酶及其上游信号分子基因表达的变化

GENE EXPRESSION OF STRESS ACTIVATED PROTEIN KINASE AND ITS MAPKS IN HYPERTROPHIC SCAR

目的 探讨增生性瘢痕和自身对照正常处皮肤中应激活化蛋白激酶 (SAPK)及其上游信号分子MAPKs(MKK4和 MKK7)基因表达的变化。 方法 提取 8例增生性瘢痕和自身对照正常处皮肤组织的总 RNA后 ,分离纯化 m RNA,用 RT- PCR方法检测 SAPK,MKK4和 MKK7基因在不同组织中的表达变化规律。 结果 增生性瘢痕中 ,MKK7和 SAPK基因表达较弱 ,自身对照皮肤组织中 ,这两种基因 PCR结果的灰度比分别为增生性瘢痕的 1.5倍和 2 .6倍 ,基因表达量明显升高 (P<0 .0 1) ,而 MKK4在这两种不同类型组织中的表达量无统计学意义 (P>0 .0 5 )。 结论 增生性瘢痕中 MKK7和 SAPK基因表达降低引起细胞凋亡减少 ,可能是瘢痕内成纤维细胞大量增殖 ,增生性瘢痕形成的机制之一。

Objective To explore the change of gene expression of stress activated protein kinase (SAPK) and its upstream signal-regulated molecule ——mitogen activated protein kinases(MAPKs) (MKK4 and MKK7) in hypertrophic scar and auto-control normal skin. Methods The total RNA was isolated from 8 hypertrophic scars and 8 auto-control skin, and then mRNA was purified. The gene expressions of MKK4, MKK7 and SAPK were examined with reverse transcription-polymerase chain reaction(RT-PCR) method. Results In hypertrophic scar, both MKK7 and SAPK genes weakly expressed. In auto-control skin, the expression of these 2 genes was significantly elevated in comparison with hypertrophic scar (P<0.01). The expression levels of these 2 genes were 1.5 times and 2.6 times as long as those of hypertrophic scar, respectively. Gene expression of MKK4 had no significant difference between auto-control skin and hypertrophic scar (P>0.05). Conclusion Decreased gene expression of MKK7 and SAPK which results in reducing cell apoptosis might be one of the mechanisms for controlling the formation of hypertrophic scar.