新城鸡瘟病毒间接血球凝集试验的初步报告

A PRELIMINARY STUDY ON THE PASSIVE HEMAGGLUTINATION TEST WITH NEWCASTLE DISEASE VIRUS

本文报导了试用同接血凝试验检查新城鸡瘟抗体的具体方法及其结果。如以“++++”号强凝集作为判定格点,则正常鸡血清的滴度不到20~×,而免疫鸡血清分别呈现40~×、80~×、320~×、640~×等滴度,而且大致与各该血清的血凝抑止价相平行。待检血清中非特异性血球凝集性物质的除去以及应用一定浓度的病毒抗原,是取得成功结果的重要条件。

Passive hemagglutinations(PH)were carried out on chicken serum samples with the NDV- sensitized tanned erythrocytes.When the highest dilution on which the strong agglutination occured was taken as the reading endpoint,the hemagglutinating titres of the four immune sera against NDV ranged from 1:40 to 1:640,while the other two normal control sera in 1:20 dilution gave negative results.Moreover,the titres of the PH corresponded in general the levels of the hemagglutination-inhibition(HI)titres of the same samples,but the identity of the PH antibody and the HI antibody had not been determined. Suitable concentration of the viral antigen,removal of non-specific agglutinins from the serum samples and incubation for 15 min.at 37℃ for absorbing the viral antigen onto tanned erythrocytes were considered to be important factors necessary for the satisfactory perform- ance of the PH reaction. The possibility of the application of the PH test on other animal viruses and virus dis- eases was suggested.