冷诱导RNA结合蛋白参与小鼠对LPS的应答

Cold-inducible RNA Binding Protein Involves in Response to LPS in Mice

旨在研究冷诱导RNA结合蛋白(CIRP)是否参与机体对LPS的应答。将48只BALB/c小鼠随机分为4组,其中1~3组为LPS刺激组,分别按体重腹腔注射2.5、5、7.5 mg·kg^(-1)的LPS,第4组为健康对照组,腹腔注射等体积生理盐水。于注射后3、6、12、18 h每组随机挑选3只小鼠,无痛处死后采取各组小鼠的心、肝、脾和肾,用本研究建立的荧光定量RT-PCR检测各组织中Cirp mRNA的转录水平,用免疫组化法研究CIRP蛋白在各组织中的表达情况。荧光定量RT-PCR检测结果显示,LPS刺激后小鼠各被检器官中CirP mRNA的转录水平显著升高,而心中Cirp mRNA的转录水平则显著降低;免疫组化试验结果显示,LPS刺激后小鼠心、肝、脾和肾中;IRP的表达量均显著增加,且有显著的时效性。本研究证实CIRP参与小鼠对LPS的应答,为鉴定CIRP的新功能提供了证据。

The objective of this paper was to explore if Cold-inducible RNA binding protein(CIRP) involved in the response of mice to LPS.Total 48 BALB/c mice were randomly divided into 4 groups,LPS was injected into abdominal cavity of each mouse in dose of 2.5,5,7.5 mg ? kg^(-1)body weight for 1-3 experimental groups.The 4th group(control group) was injected with the same volume saline solution in the same way with the above groups.Three mice were selected in each group and euthanized at 3,6,12 and 18 h post injection.The heart,liver,spleen and kidney of each mouse were collected.Real-time RT-PCR established in this study and immunohistochemistry were used to detect the transcription of CIRP mRNA and expression of CIRP protein in each organ.The results showed that the Cirp mRNA significantly increased in liver,spleen and kidney post LPS injection,while Cirp mRNA transcription in heart was inhibited at all time points.CIRP protein was highly expressed in all detected organs after LPS stimulation.In conclusion,the results in this paper demonstrated that CIRP involves in response to LPS in mice and present evidence to identify new feature of CIRP.