血管活性肠肽在中国黄羽鹌鹑法氏囊中的定位

Immunohistochemical Localization of Vasoactive Intestinal Peptide in Bursa of Fabricious of Chinese Yellow Feather Quails

本文采用免疫组化SABC法和显微图像分析技术对不同周龄中国黄羽鹌鹑法氏囊中VIP进行组织学定位,观察在不同生长阶段VIP的分布规律及形态特征。结果显示,VIP在新生雏和1、3、5、9、13、17、21、23、25、32、36周龄中国黄羽鹌鹑法氏囊中广泛存在,多呈长梭形、马蹄形、椭圆形、团块状或细小颗粒状,主要分布在黏膜下层、肌层、浆膜,其中以3周龄的分布最为典型。到25周龄时VIP阳性反应的平均光密度值达到最大,随后逐渐减弱。结果表明,VIP存在于各生长阶段的中国黄羽鹌鹑法氏囊中,其分布规律与法氏囊的发育及功能变化有一定的相关性。

The distributional characteristics of VIP in different ages bursa of Fabricious of Chinese yellow feather quails were observed by using the immunohistochemical SABC method and microscopic image analysis.The result showed that VIP widely exists at newborn,1,3,5,9,13,17,21,23,25,32,36-week-old of Chinese yellow feather quail bursa,formed a long fusiform,horseshoeshaped,oval,lumps or fine granular,mainly distributed in the submucosa.muscularis,serosa,and the distribution is the most typical at 3 weeks of age.To 25 weeks of age,the average optical density of VIP positive reaction reached the maximum,then gradually weakened.The result proves that VIP exists at all stage of growth in Chinese yellow feather quails bursa,and with the development and function of changes in bursal characteristics showing a certain correlation.