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柔嫩艾美耳球虫感染对京海黄鸡不同组织中Zyxin、CD4和TNFRSF1A基因mRNA表达的影响 被引量:1

The Effect of Emeria tenella Infection on the Expression of Zyxin,CD4,TNFRSF1A in Different Tissues of Jinghai Yellow Chicken
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摘要 为了探讨Zyxin、CD4、TNFRSF1A 3个球虫抗性相关基因在雏鸡感染柔嫩艾美耳球虫后表达量的变化,本研究以京海黄鸡为试验动物,对感染柔嫩艾美耳球虫雏鸡的10个组织进行了荧光定量分析,结果发现,Zyxin、CD4、TNFRSF1A基因在感染柔嫩艾美耳球虫后的雏鸡多数组织中表达量变化较大。感染组Zyxin基因在腺胃、小肠等组织表达量较高,除肺、肝、肾和心肌组织外,Zyxin基因表达量均极显著高于对照组(P<0.01);感染组雏鸡CD4基因在脾、肺、胸腺等免疫器官中的表达量极显著高于对照组(P<0.01);感染后TNFRSF1A基因在小肠和盲肠中表达量最高,且感染组小肠、盲肠、脾、腺胃和胸腺中的表达量与对照组差异也极显著(P<0.01)。但法氏囊中3个基因的表达量均较低,且仅Zyxin基因差异显著,提示法氏囊在球虫感染过程中可能并未起到重要作用。鸡柔嫩艾美耳球虫主要寄生在盲肠组织中,Zyxin和TNFRSF1A基因在感染后的盲肠组织中表达量的极显著升高(P<0.01),说明这2个基因与鸡球虫的感染关系密切,可能在雏鸡感染过程中起重要作用。 In order to analyze the effect of Emeria tenella infection on the expression of Zyxin,CD4 and TNFRSFIA in Jinghai Yellow chickens,the expression of the three genes in 10 tissues were detected by fluorescent quantitative PCR.The results showed that the expression of the 3genes were significantly different between the infected and control groups in most tissues.Zyxin expression in glandular stomach was the highest among detected tissues in infected group,except for in lung,liver,kidney and cardiac muscle,the expression of infected group was significantly higher than that of control group(P<0.01);CDA expression of infected group in some immune organs,such as spleen,lung and thymus,was significantly higher than that of control group(P<0.01);TNFRSF1A expressions in small intestine and caecum were at high level,and was significantly higher in infected group than that in control group(P<0.01) in small intestine,caecum,spleen,glandular stomach and thymus gland;Three gene expressions in bursa of Fabricius were at low level,and none significant difference was found between the infected and control groups except for Zyxin,which suggested that bursa of Fabricius probably did not play an important role in the infection of Emeria tenella.In caecum,the parasitic site of Emeria tenella,the expression of Zyxin and TNFRSFIA of infected group had significant difference with the control group(P<0.01),which indicate that the two genes had a close relationship with the infection of Emeria tenella.
出处 《畜牧兽医学报》 CAS CSCD 北大核心 2014年第8期1369-1374,共6页 ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金 国家肉鸡产业技术体系项目(nyctx-42-G1-05) 江苏省高校自然科学研究重大项目(11KJA230001) 江苏高校优势学科建设工程项目(PAPD) 江苏省产学研联合创新资金前瞻性联合研究项目(BY2013063-06) 扬州大学新世纪创新工程
关键词 感染 柔嫩艾美耳球虫 基因表达 京海黄鸡 infection Emeria tenella gene expression Jinghai Yellow chicken
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