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CRISPR/Cas9系统在基因表达调控中的应用 被引量：4

Application of CRISPR/Cas9 System for Gene Regulation
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摘要 CRISPR/Cas9系统是2012年发展起来由RNA导向的基因组编辑技术。相比ZFN和TALENs技术,CRISPR/Cas9系统具有类似的打靶效率,且更易于操作。通过将核酸酶Cas9蛋白D10A和H840A位点突变,可以获得失去切割活性的dCas9蛋白。借助导向RNA将dCas9与DNA特定位点结合的作用,可以实现抑制或激活基因转录,从而达到对特定基因表达进行调控的目的。本文主要从作用原理、影响因素等方面综述了CRISPR/Cas9系统在基因调控中的应用,以期为相关研究提供参考。 CRISPR/Cas9 system is one of genome editing technologies developed in 2012.Compared with TALENs and ZFN technologies,CRISPR/Cas9 system has similar gene editing efficiency but is easily manipulated.The engineered dCas9 protein without endonuclease catalytic activity can be obtained by mutating D10 Aand H840Asites of Cas9 protein.The dCas9 guided by gRNA can be targeted to specific DNA site for the purposes of either inhibiting or activating gene transcription.This paper reviewed the application of CRISPR/Cas9 system in gene regulation as regarding the principle and affecting factors and so on,in order to provide a reference for related research.
作者 魏泽辉 贾存灵 张智英
机构地区 西北农林科技大学
出处 《畜牧兽医学报》 CAS CSCD 北大核心 2014年第9期1387-1392,共6页 ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金 国家自然科学基金资助项目(U1036604 31172186)
关键词 CRISPR dCas9 转录抑制 转录激活 CRISPR dCas9 transcription repression transcription activation
分类号 Q78 [生物学—分子生物学]
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参考文献10

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共引文献3

同被引文献53

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畜牧兽医学报
2014年 第9期

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