饲料样品储存条件对仿生消化率测值的影响

Effect of Different Storage Conditions on the in vitro Digestibility of Feedstuffs Using a Computer-controlled Simulated Digestion System

本试验旨在探讨不同储存温度、包装方法和储存时间对仿生消化系统测试玉米和豆粕样品的干物质消化率和酶水解物能值的影响,为仿生消化系统测试饲料样品的储存方法提供依据。试验采用3×2×7三因素裂区试验设计,主区处理为储存温度(A因素),设2个水平,即4和-20℃;副区处理为包装方法(B因素)和储存时间(C因素),其中包装方法(B因素)设3个水平,即普通自封袋包装、抽真空包装、抽真空后充氮包装,储存时间(C因素)设7个水平,即0、2、4、6、8、10、12个月;每个处理3个重复,测定各处理下玉米和豆粕样品的干物质消化率和酶水解物能值。结果表明:1)-20℃条件下,玉米和豆粕样品的干物质消化率和酶水解物能值显著高于4℃条件下的相应测值(P<0.05);与初始测值相比,-20℃条件下的相应测值具有较小的变异性。2)在抽真空包装和抽真空后充氮包装方法下,玉米和豆粕样品的干物质消化率显著高于普通自封袋包装的相应测值(P<0.01);玉米的酶水解物能值显著高于普通自封袋包装的相应测值(P<0.01);但包装方法不影响豆粕酶水解物能值(P>0.05);与初始测值相比,抽真空后充氮包装方法相应测值的变异性最小。3)随着储存时间的延长,玉米和豆粕样品的干物质消化率和酶水解物能值显著性降低(P<0.01)。玉米和豆粕样品的干物质消化率和酶水解物能值受储存温度、包装方法和储存时间的影响,在-20℃抽真空后充氮气储存方法下,玉米和豆粕样品在12个月内干物质消化率和酶水解物能值变异较小,能起到较好的储存效果。

The effects of storage temperature,packing methods and storage time on the in vitro dry matter digestibility(DMD)and enzymatic hydrolysate gross energy(EHGE)of corn and soybean meal were investigated to provide the basis for the storage of feed samples of computer-controlled simulated digestion system(CCSDS).A 3×2×7factorial arrangement in a Split plot design was used.The main plot was storage temperature at 4or-20 ℃ and the sub-plots were packing methods with a zip-lock bag,vacuum package or vacuum &nitrogen filling package and storage time at 0,2,4,6,8,10 or 12months.The DMD and EHGE of corn and soybean meal were determined by using a computer-controlled simulated digestion system.The results showed as follows:1)The DMD and EHGE of corn and soybean meal stored at-20℃ were significantly greater than that stored at 4 ℃(P<0.05).Compared with the initial values,the variability of the DMD and EHGE of corn and soybean meal stored at-20℃ were less than that stored at 4℃.2)The DMD of corn and soybean meal stored in the vacuum package or in the vacuum &nitrogen filling package were significantly greater than that stored in the zip-lock bag(P<0.01).The EHGE of corn stored in the vacuum package or in the vacuum &nitrogen filling package were significantly greater than that stored in the zip-lock bag(P<0.01).Compared with the initial values,the variability of the DMD and EHGE of corn and soybean meal stored in the vacuum &nitrogen filling package were less than that stored in the other packing methods.3)The DMD and EHGE of corn and soybean meal decreased as the storage time increased from 0to 12months(P<0.01).In conclusion,the DMD and EHGE of corn and soybean meal were affected by the storage temperature,packing methods and storage time.The DMD and EHGE of corn and soybean meal stored in the vacuum &nitrogen filling package at-20℃had low variability and good storage effect.