miR-M7-5p靶向调控马立克病病毒原癌基因meq的表达

Marek's Disease Virus Oncogene meqis Targeted by the Viral microRNA miR-M7-5p

马立克病病毒(MDV)基因组编码数十个miRNA,并且可能在MDV的感染及致病过程中发挥重要作用。此前研究发现,MDV-1编码的miR-M7-5p在病毒感染宿主发病的过程中具有高水平表达特征,为探讨miR-M7-5p对MDV可能具有的自我调控作用,利用生物信息学方法对MDV编码的蛋白基因进行了扫描分析,预测发现了10个潜在的miR-M7-5p结合靶点,其中包括MDV的原癌基因meq。双荧光报告试验表明miR-M7-5p与meq基因的3′-UTR在体外能够相互作用,qRT-PCR进一步证实miR-M7-5p在体内能够下调meq基因mRNA的转录。结果表明,miR-M7-5p能够自我调控病毒原癌基因meq的表达,在MDV致瘤过程中可能发挥重要作用。

In the viral genomes of Marek's disease virus(MDV),dozens of miRNAs have been identified and some of them are suggested to potentially play important roles in MDV pathogenesis and oncogenesis.Previous studies have shown that the MDV-1-encoded miR-M7-5p is expressed at a high level during different phases of the developing disease.To investigate the potential self-regulation of miR-M7-5p on MDV during virus infection,we have presently scanned the MDV protein-coding genes utilizing bioinformatics method and obtained 10 potential target candidates for miR-M7-5p,including the most important viral oncogene meq.Dual luciferase reporter assay(DLRA)showed that miR-M7-5p can interact with the 3′-UTR of meq gene.Using realtime quantitative RT-PCR,we further confirmed that the transcription level of meqgene in MDVinfected CEF was down regulated by miR-M7-5p.Our data suggests that the oncogene meqis selfregulated by the viral miRNA miR-M7-5p in the infection processes,which may contribute toMDV oncogenesis.