钼对镉胁迫下山羊红细胞膜抗氧化功能和肝XOD基因转录的影响

Effects of Molybdenum under Stress of Cadmium on Red Blood Cell Membrane Antioxidation and XOD Gene Transcription of Liver in Goat

旨在观察钼对镉胁迫下山羊红细胞膜抗氧化功能和肝XOD基因转录的影响。选取体重约为20kg的山羊36只分为4组(每组3个重复,每个重复3只):试验组灌服相同量的CdCl2和不同量的[(NH4)6Mo7·O24·4H2O],对照组灌服对应量去离子水,试验期50d。具体分组为对照组(Cd 0mg·kg-1+Mo 0mg·kg-1)、试验Ⅰ组(Cd0.5mg·kg-1+Mo 15mg·kg-1,按体重计算,下同)、试验Ⅱ组(Cd 0.5mg·kg-1+Mo 30mg·kg-1)、试验Ⅲ组(Cd 0.5mg·kg-1+Mo 45mg·kg-1)。于试验的第0、10、20、30、40、50天每组山羊采血,于试验的第0、25、50天每组山羊取肝,测定相关指标。结果表明,随着试验时间的延长,各试验组红细胞膜XOD、LDH活性呈现上升的趋势,ATPase、ALP活性呈现下降的趋势,并在第50天时,与对照组相比,各试验组红细胞膜XOD、LDH活性显著上升(P<0.01),ATPase、ALP活性显著降低(P<0.01),T-AOC无统计学意义(P>0.05)。随着试验时间的延长,各试验组肝XOD基因转录量呈下降的趋势,并在第50天时,与对照组相比,各试验组XOD基因转录量显著下降(P<0.05)。镉胁迫下随着钼水平的升高红细胞膜抗氧化功能降低,肝XOD基因的转录量下调,钼与镉呈协同关系。

This experiment was conducted to determine effects of molybdenum under stress of cadmium on red blood cell membrane antioxidation and XODgene transcription of liver in goat.Thirty-six goats weighing approximately 20 kg each were randomly allocated into 4groups of 9goats each(three replicates every group).Goats of experiment group were drenched the same amount CdCl2 and the different amount [(NH4)6Mo7O24 ·4H2O],and goats of control group were drenched corresponding quantitative deionized water.Groups consisted of control group(0Cd+0Mo),experiment groupⅠ(Cd 0.5mg+Mo15mg,per kg of body weight),experiment groupⅡ(Cd0.5mg+Mo30mg,per kg of body weight),experiment groupⅢ(Cd0.5mg+Mo45mg,per kg of body weight).Blood was collected on the 0th,10 th,20th,30 th,40th and 50 th day and liver were collected on the 0th,25 th and 50 th day in experiment period from every group,then relatedindexes were determined.Results showed that XOD,LDH in red cell membrane of each experiment group were significantly raised,reduced ATPase,ALP activity were observed following the increase of experiment time,in addition,compared with control group,levels of XOD,LDH from each group were significantly higher while ATPase,ALP were lower on 50 th day(P<0.01),TAOC had no statistical significance(P>0.05);XODtranscription of liver in each experiment group showed a time-dependent effect,in addition,compared with control group,transcription of XODsignificantly descend on 50 th day(P<0.05).These results showed that red blood cell antioxidation decreased and XODtranscription of liver downregulated with the increasing of molybdenum under stress of cadmium in goats,and there was synergistic relationship between molybdenum and cadmium.