摘要
本研究以抗病力强的华北型黑猪大蒲莲猪和抗病力相对较弱的引进猪种长白猪的断奶仔猪为研究对象,利用全自动血液细胞分析仪和流式细胞仪测定了大蒲莲(124头)和长白(187头)仔猪血液的血常规和T淋巴细胞亚群性状,并对这些性状进行了品种和母猪效应分析。结果表明:大多数血常规性状的变化范围较小(平均22.92%),成熟T淋巴细胞各性状的变异系数(平均44.5%)小于非成熟T淋巴细胞各性状的变异系数(平均112.77%)。大蒲莲和长白2个品种间比较,除RBC、HGB、LY、MID和PLT外,其他14个血常规性状的差异均达到极显著(P<0.01)水平;而对于T淋巴细胞亚群性状,除CD4+CD8-CD3-、CD4+CD8+CD3-和CD3+外,其他7个性状的差异均达到显著(P<0.05)或极显著水平(P<0.01)。本研究中的124头大蒲莲仔猪和187头长白仔猪分别来自13窝大蒲莲母猪和28窝长白母猪,母猪窝效应对血常规各性状均具有显著(P<0.05)或极显著(P<0.01)的影响。本研究大规模测定了大蒲莲猪和长白猪的仔猪血常规和T淋巴细胞亚群各指标,为了解中外不同猪种的差异,挖掘我国地方猪的种质特性奠定基础。
In the present study,Dapulian piglets(n=124),one indigenous breeds with higher disease resistance of North China,and Landrace(n=187),one commercial breed with lower disease resistance were used,routine blood traits and T-lymphocyte subpopulation traits of these piglets were determined by automatic blood cell analyzer and flow cytometry.Additionally,we performed analyses of breeds and litter effect on these traits in piglets.The results indicated that the most of the blood routine traits had small variation range,with the average variation coefficient of22.92%,while the variation coefficient of mature T-lymphocyte cells(with average of 44.5%)were smaller than those of immature ones(with average of 112.77%).Comparison between the two breeds,except for RBC,HGB,LY,MID and PLT,the other 14 traits of routine traits were significantly different at 0.01 level.As respective for the T-lymphocyte subpopulation traits,except for CD4+CD8-CD3-,CD4+CD8+CD3- and CD3+,the other 7traits were significantlydifferent at 0.05 or 0.01 level.The piglets used in the study were sampled from 13 brood Dapulian sows and 28 brood Landrace sows,and analyses indicated that the sow effects on all the hematological traits were all significant at 0.05 or 0.01 level.To our knowledge,this is the first study aimed at determining and analyzing the routine blood traits and T-lymphocyte subpopulation traits of Dapulian and Landrace piglets at population level.Results of the study may provide important information for understanding the difference between the indigenous and commercial breeds and mining the characteristic of indigenous breed.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2014年第11期1760-1766,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(31201779)
转基因生物新品种培育科技重大专项(2014ZX08009-045B)
山东省农业产业技术体系生猪创新团队建设项目(SDAIT-06-011-03)
山东省农业良种工程农业生物资源创新利用研究“山东地方猪抗病抗逆基因的发掘及种质创新研究”
关键词
大蒲莲猪
长白
血细胞性状
血常规
T淋巴细胞亚群
Dapulian pigs
Landrace
hematological traits
blood routine trait
T-lymphocyte subpopulation trait