北京油鸡肌肉和卵巢组织基因组DNA甲基化状态检测与分析

Detection of DNA Methylation in Whole Genomes of Muscle and Ovary Tissues of Beijing You Chicken

以17周龄北京油鸡为试验材料,应用甲基化敏感扩增多态性(Methylation sensitive amplified polymorphism,MSAP)方法检测肌肉和卵巢组织的基因组DNA甲基化模式和程度,进而分析肌肉和卵巢组织基因组DNA甲基化的差异。结果表明,应用12对选择性扩增引物共产生4 297条清晰可辨的扩增条带,平均每个个体每对引物组合可获得10~20个条带。在检测的个体中,肌肉组织的甲基化水平为35.86%,卵巢组织为31.50%,2种组织的甲基化水平差异显著(P<0.01)。北京油鸡体重与肌肉组织的甲基化水平存在极显著负相关(P<0.01),与卵巢组织的甲基化水平的相关性未达到显著水平(P>0.05)。分离鉴定了5个组织特异性的甲基化片段。结果显示,北京油鸡肌肉和卵巢组织的基因组DNA的甲基化状态存在差异,而肌肉组织的甲基化状态对北京油鸡的体重具有重要作用。

The DNA methylation extent and pattern was assessed in genomes in muscle and ovary tissues from the 17-week-old Beijing You chicken using a methylation sensitive amplified polymorphism(MSAP)approach in this study.We analyzed different DNA methylation levels in different tissues.A total of 4 297 clear bands were obtained using 12 pairs of selective amplification primers,about 10-20 bands per sample with a pair of primers.The degree of methylation was approximate 35.86% in muscle and 31.50% in ovary in Beijing You chickens,and a significant difference was found between 2tissues at the methylation levels(P<0.01).Further analysis indicated that no significant relationship was detected in ovary tissue between the methylation levels and individual weight(P>0.05),but strongly negative correlation was depicted in muscle tissue(P<0.01).Five tissue-specific MSAP fragments were isolated and verified.Overall results reveal that there is a significant difference at the methylation levels between muscle and ovary tissues,and the methylated state of muscle tissue may play an important role in the body weight of Beijing You chicken.