单色光及松果体摘除对肉鸡肝抗氧化功能的影响

Effect of Monochromatic Light and Pinealectomy on Antioxidant Function of Liver in Broilers

本研究旨在探究单色光对肉鸡肝抗氧化功能的影响和褪黑激素的介导作用。选择120只0日龄AA肉鸡随机分为4组,分别饲养于白、红、绿和蓝光下(n=30),3日龄时按光色条件每组随机选取20只鸡分别施行松果体摘除手术(n=10)和假手术(n=10),剩余鸡为对照组,每组同条件饲养至14日龄时利用抗氧化试剂盒检测各光色不同处理组肉鸡肝抗氧化水平。结果显示,饲养于绿光下对照组和假手术组的肉鸡肝T-SOD、GSH-Px、T-AOC的水平显著高于其他光组(7.82%~46.33%,P<0.05),而MDA含量最低;松果体摘除阻断褪黑激素分泌,各处理组肉鸡肝抗氧化酶活性均降低(12.64%~38.50%),而MDA含量升高。以上结果说明,单色光能够影响肉鸡肝的抗氧化功能,其中绿光显著增强肝抗氧化功能;而摘除松果体后不同光色环境下的肝抗氧化能力减弱显著。提示褪黑激素参与了单色光影响肉鸡肝的抗氧化功能。

The present study was aimed to explore the effect of monochromatic light on antioxidant function in liver and the role of melatonin in broilers.One hundred and twenty post-hatching day(P0)male Arbor Acres(AA)broilers were divided into four groups randomly and reared under the white,red,green and blue light environments(n=30).Twenty broilers from each light group was chosen randomly for pinealectomy surgery(n=10)and sham operation(n=10)at P3,and the remaining broilers were as the control group.Antioxidant assay kits were used to detect the antioxidant level in liver of broilers in different light groups reared under same environment to P14.The results showed that the T-SOD,GSH-Px,T-AOC level in liver of broilers reared under the green light in control and sham-operation groups were higher(7.82%-46.33%,P<0.05)than other various light groups,while the content of MDA was the lowest.After pinealectomy blocked the secretion of melatonin,the activity of antioxidant enzymes were reduced(12.64%-38.50%)and MDA contents were increased in various light groups.These results showed that the monochromatic light could influence the antioxidant level of liver in broilers,and green light enhanced the antioxidant function of liver.However,pinealectomy could significantly reduce the liver antioxidant level in various light groups.These results suggested that melatonin was involved in the effect of monochromatic light on antioxidant function in liver of broilers.