氟对小鼠脾淋巴细胞ERK通路相关基因及蛋白质表达的影响

Effects of Fluoride on the Expression of ERK Signaling Pathway Associated Gene and Proteins in the Splenic Lymphocytes of Mouse

为探讨氟化钠对小鼠脾淋巴细胞细胞外信号调节蛋白激酶(ERK)通路相关基因及蛋白表达的影响,将体内不同氟质量浓度染毒鼠的脾淋巴细胞和体外不同氟浓度作用的脾淋巴细胞经过细菌脂多糖(LPS)刺激培养后,采用Real-time PCR和Western blotting检测ras、c-raf、MEK1、ERK1在mRNA水平的转录变化以及ERK1在蛋白质水平的表达差异。结果显示:小鼠脾淋巴细胞ERK通路的激活可被低质量浓度的氟抑制,但随氟质量浓度增加,其抑制趋势逐渐减少而转为促进。表明氟化物可通过影响小鼠脾淋巴细胞ERK通路的激活,从而进一步影响免疫系统的功能。

The current study was undertaken to study the effects of fluoride on the expression of ERK signaling pathway associated gene and proteins in the splenic lymphocytes of mouse.The splenic lymphocytes of the different level of fluoride-treated mouse in vivo and the splenic lymphocytes with the different level of sodium fluoride in vitro were cultured in RPMI-1640 medium with bacterial lipopolysaccharide,then the mRNA level of ras/c-raf/MEK1/ERK1 and the protein expression of ERK1 were detected using the Real-time PCR and Western blotting.The results showed that activation of the ERK pathway in mouse splenic lymphocytes can be inhibited by fluoride in low concentrations,but with increasing fluoride concentration,the degree of inhibition was reduced,ERK pathway activation was turn into promoting.These results suggested that fluoride can affect the activation of ERK pathway in mouse splenic lymphocytes,thus further influence the function of the immune system.