小型猪专用复合麻醉剂及赛拉嗪对大鼠不同脑区c-jun蛋白表达的影响

Effects of the Combined Anaesthetic for Miniature Pigs and Xylazine on the c-jun Protein Expression in Different Encephalic Region of Rats

为研究小型猪专用复合麻醉剂(XFM)及赛拉嗪对大鼠不同脑区c-jun蛋白表达的影响,将114只健康SD大鼠,随机分为空白对照组(B组,n=6)、生理盐水对照组(C组,n=36)、XFM麻醉组(H组,n=36)和赛拉嗪组(X组,n=36),B组不给药,C组腹腔注射生理盐水,H组腹腔注射XFM麻醉,X组腹腔注射与XFM合剂中相同用量的赛拉嗪。除B组外,C、H、X组分为6个亚组:麻醉诱导期(Ⅰ期)、翻正反射消失(Ⅱ期)、翻正反射消失后15min(Ⅲ期)、翻正反射消失后35min(Ⅳ期)、翻正反射恢复(Ⅴ期)、恢复直线爬行(Ⅵ期)。各组大鼠在对应的时间点断头处死,分离大脑皮层、海马、小脑及脑干,并提取各脑组织蛋白质,用免疫印迹法检测各脑组织样品c-jun蛋白含量。结果表明:与B组相比,(1)C组大鼠大脑皮层、海马、小脑及脑干c-jun蛋白表达无显著变化(P>0.05);(2)H组上述脑区c-jun蛋白表达量上升,Ⅵ期表达下降;(3)X组上述脑区c-jun蛋白表达量也上升,后期表达量下降,且与H组相比,X组c-jun蛋白表达量较低。各脑区间,c-jun蛋白表达相比差异不显著(P>0.05)。结果提示:XFM和赛拉嗪麻醉过程中,大鼠大脑皮层、海马、小脑及脑干c-jun蛋白表达量随麻醉加深而增加,在麻醉苏醒过程中,c-jun蛋白表达量下降,提示XFM和赛拉嗪可以诱导上述脑区c-jun蛋白表达上调,这些脑区c-jun蛋白表达的改变可能是XFM和赛拉嗪作用机制之一。

The purpose of this study was to investigate the effects of miniature pig combined anaesthetic(XFM)and xylazine on c-jun protein expression in different encephalic regions of rat.One hundred and fourteen SD rats were randomly divided into blank control group(B group,n=6),saline solution control group(C group,n=36),XFM group(H group,n=36)and xylazine group(X group,n=36).Rats in B group were raised normally without any treatment,rats in C group were injected with normal saline,and rats in H group,X group were received XFM,Xylazine treatment,respectively.Each group was divided into six subgroups except B group:anesthesia induction stage(stageⅠ),disappear stage of righting reflex(stageⅡ),15 min after righting reflex disappear(stage Ⅲ),35 min after righting reflex disappear(stage Ⅳ),recovery stage of righting reflex(stageⅤ)and recovery of linear crawling(stage Ⅵ).Rats were decapitated in relative time,then cerebral cortex,hippocampus,cerebellum and brainstem tissue were separated.Proteins were extracted from these brain tissues,and c-jun protein content was detected by Western blot.Results showed that:(1)In C group,c-jun protein expressions had no significant changes in cerebral cortex,hippocampus,cerebellum and brainstem of rats(P>0.05)compared with B group;(2)In H group,c-jun protein expression increased in these encephalic regions,and decreased in stageⅥ(P<0.01)compared with B group;(3)In X group,c-jun protein expression increased in these encephalic regions(P<0.01),and decreased in late stages(P<0.01)compared with B group.And c-jun protein expression level of X group was lower than that of H group(P<0.01).(4)c-jun protein expressions in these encephalic regions had no significant difference(P>0.05).During rats anesthetized with XFM and xylazine,c-jun protein expression level increased in cerebral cortex,hippocampus,cerebellum and brainstem tissue with the deepening of anaesthesia induction,and decreased when recovery from anesthesia.The results suggest that the expression changes of c-jun protein induced by XFM and xylazine in the encephalic regions may be one of the anesthesia mechanisms.