Kiss-1基因多态性与苏淮山羊产羔数的关联分析

Kiss-1 Gene Polymorphisms and Its Association with Litter Sizes in Suhuai Goats

为了研究Kiss-1基因多态性与苏淮山羊产羔数之间的关系,本研究采用DNA池测序技术筛选苏淮山羊Kiss-1基因突变位点,采用AS-PCR和PCR-RFLP方法检测130只苏淮山羊Kiss-1基因突变位点的多态性,并分析其与产羔数的关联性。结果表明,在苏淮山羊内含子1中发现2个突变位点g.2270C>T和g.2510A>G,其中g.2510A>G位点有3种基因型AA、AG和GG,基因型频率分别为0.209、0.496和0.295;g.2270C>T位点在苏淮山羊中共检测3种基因型(CC、CT和TT),基因型频率分别为0.143、0.750和0.107。关联分析发现,g.2510A>G位点3种基因型在苏淮山羊各胎产羔数和平均产羔数间差异不显著,g.2270C>T位点中CC型的二胎产羔数显著高于CT和TT型(P<0.05)。单倍型分析发现,g.2510A>G和g.2270C>T位点在苏淮山羊群体中共构建8种单倍型,其中AGCT是主要的单倍型;各单倍型与苏淮山羊产羔数的关联分析发现仅AGCT单倍型的头胎产羔数与AACT单倍型的头胎产羔数间差异显著,其他单倍型在苏淮山羊的各胎产羔数间差异均不显著。综合以上研究表明,Kiss-1基因的g.2270C>T位点可能对苏淮山羊繁殖性状的选育具有一定的指导作用。

To investigate the effects of the polymorphisms of Kiss-1gene on the reproductive traits of Suhuai goats,the mutations of Kiss-1gene were screened using DNA pool technology,the polymorphism of Kiss-1gene in 130 Suhuai goats were detected using AS-PCR and PCRRFLP methods and its correlation with the litter size of Suhuai goats was analyzed.The results showed that 2SNPs of g.2270C>T and g.2510A>G were found in Kiss-1gene intron 1sequence of Suhuai goats.Three genotypes(AA,AG and GG)were found at g.2510A>G locus in Suhuai goats,and the genotype frequency were 0.209,0.496 and 0.295,respectively;at g.2270C>T locus,3genotypes(CC,CT and TT)were detected in Suhuai goats and the genotype frequency were 0.143,0.750 and 0.107,respectively.Association analysis showed that there was no significant difference among 3genotypes(AA,AG and GG)of Suhuai goats in each parity litter size and average litter size at g.2510A>G locus;at g.2270C>T locus,the second parity littersize of CC genotype was significantly higher than those of CT(P<0.05)and CC genotypes(P<0.05).Haplotype analysis found 8haplotypes and the first parity litter size of AGCT haplotype was significantly higher than that of AACT haplotype,and there existed no significant difference in litter size of Suhuai goats among other haplotypes.Therefore,it can be concluded that g.2270C>T locus of Kiss-1gene may be a candidate molecular marker for reproductive traits selection in Suhuai goats.