双峰驼CYP基因家族的比较研究

Comparative Study of CYP Gene Family in Bactrian Camel(Camelus bactrianus)

为探讨双峰驼(Camelus bactrianus)物质代谢途径的特殊性和独特的解毒能力是否与其CYP基因家族的特殊性有关,本试验在对双峰驼全基因组测序数据进行系统分析的基础上,采用基因注释及比较基因组学研究方法,对双峰驼CYP基因家族进行了分析。结果显示,在双峰驼基因组中首次发现63个CYP基因拷贝,分别属于17个CYP家族和38个亚家族;其中,有9个为多基因家族,且CYP2、CYP3和CYP4分别有27、6和7个成员,分别占双峰驼全部CYP基因的43%、10%和11%。与牛、马、鸡和人的CYP基因相比,双峰驼CYP基因亚家族的分布明显不同,在拷贝数上拥有较多的CYP2J和CYP3A。因此,双峰驼特有的某些生物学特性和代谢途径与其高拷贝数的CYP2J和CYP3A基因有一定关系,分析结果为进一步揭示双峰驼耐高盐和独特的解毒能力奠定了基础。

In order to investigate whether the Bactrian camel's special metabolic pathways and unique detoxic capabilities were caused by particularities of CYP gene family,the Bactrian camel's whole genome sequencing data were systemically analyzed and annotated,and then CYPgene family was searched from the whole protein database,and compared with CYPgene families of cattle,horse,chicken and human,respectively.The results showed that the total of 63 CYPgene copies were found in Bactrian camel's whole genome,and were classified into 17 families and 38 subfamilies.Among them,9multi gene families were found,and CYP2,CYP3 and CYP4had 27,6and 7subfamilies,accounting for 43%,10%and 11%in camel CYP,respectively.Compared with cattle,chicken,horse and human,the distribution of CYP gene subfamilies in camel was different,with more CYP2 Jand CYP3Acopies in Bactrian camel,which was probably associated with Bactrian camel's some specific biological characteristics and metabolic pathways.Therefore,the certain special biological characteristics and metabolic pathways of Bactrian camels are related to their high copy number of CYP2 Jand CYP3Agenes,and the present analytical results will lay agood foundation for further revealing of Bactrian camel's unique properties of salt tolerance and detoxification.