小型猪复合麻醉剂对大鼠中枢神经系统p-p38MAPK蛋白表达的影响

Effects of the Combined Anaesthetic for Miniature Pigs on the Expression of p-p38MAPK in Central Nervous System of Rats

为研究小型猪复合麻醉剂(XFM)对大鼠中枢神经系统p-p38蛋白表达的影响,将30只大鼠分为对照组(C组)和麻醉剂组(M组),M组又分为4个亚组:M1组(注射XFM后大鼠翻正反射消失即刻)、M2组(注射XFM后大鼠翻正反射消失后1h)、M3组(注射XFM后大鼠翻正反射恢复即刻)和M4组(注射XFM后大鼠翻正反射恢复后1h),各组大鼠到达预定的时间点后分别采取脑组织,应用RT-PCR法检测脑内p38 mRNA转录量,应用Western blot方法检测中枢神经系统中p-p38蛋白的表达量。大鼠注射XFM后,与对照组比较,试验组大鼠大脑皮层和丘脑p38mRNA转录量显著降低(P<0.05),在苏醒过程中有所恢复,但仍显著低于对照组(P<0.05);大脑皮层和丘脑内p-p38蛋白的相对表达量,在M1、M2组的时间点显著低于对照组(P<0.05或P<0.01);大鼠注射XFM后,与对照组比较,试验组大鼠小脑、海马、脑干内p38mRNA转录量显著升高(P<0.05),在苏醒过程中仍显著高于对照组(P<0.05);小脑、海马、脑干内p-p38蛋白的表达量,在M1组、M2组、M3组显著升高,与对照组相比差异显著(P<0.05或P<0.01),在M4组表达量下降,与对照组相比差异不显著(P>0.05)。结果表明,XFM诱导大鼠大脑皮层、丘脑内p38 mRNA及p-p38蛋白表达下调,p38 mRNA及磷酸化蛋白的改变可能是XFM作用的机制之一。

In order to investigate the expression of p-p38 in central nervous system in rats anesthetized with the Combined Anaesthetic for Miniature Pigs(XFM),30 rats were divided randomly into two groups:control group and XFM group.XFM group consisted of four subgroups:M1subgroup(rats received XFM intraperitoneally and then waiting the disappearance of right reflection);M2subgroup(rats received XFM intraperitoneally and then waiting for 1hafter the disappearance of right reflection);M3subgroup(rats received XFM intraperitoneally and then waiting the recovery of right reflection)and M4subgroup(rats received XFM intraperitoneally and then waiting for 1hafter the recovery of right reflection).The brain tissues were taken when reaching each time point.With the method of RT-PCR,expression of p38 mRNA were detected,and the expression of p-p38 in central nervous system was detected with Western blot.The results showed that,compared with the control group,the transcription of p38 mRNA on cerebellum and thala-mus in experiment groups were significant lower than that in control group(P<0.05),and p38 mRNA increased at group M3 and group M4,but significantly lower than control group(P<0.05).In group M1 and group M2,the expression of p-p38 in cerebellum and thalamus were significantly lower than that in the control group(P<0.05),but the expression of p-p38 in cerebellum,hippocampus,brainstem increased significantly after administration of XFM(P<0.05)in group M1,group M2,and group M3,compared with the control group,and in group M4,the expression of p-p38 decreased,were no difference with the control group(P>0.05).These results indicated that the decrease of the expression of p-p38 in cerebellum and thalamus were induced by XFM,and these changes of p38 mRNA and p-p38 maybe one of the anaesthetic mechanisms of XFM.