藏鸡Chemerin和ChemR23基因时序表达及其与肌内脂肪含量的相关性研究

Association Analysis of Sequential Expression of Chemerin and ChemR23 with Intramuscular Fat Contents in Tibetan Chicken

本试验旨在阐明藏鸡Chemerin及其受体ChemR23基因的组织和时序表达谱,分析这两个基因mRNA表达水平对肌内脂肪沉积的影响。以孵化0~210日龄的藏鸡为材料,采用RT-PCR方法克隆Chemerin与ChemR23基因,利用荧光定量PCR(qPCR)技术检测两个基因在不同组织及不同发育阶段的表达情况,并将基因表达量与肌内脂肪含量进行相关分析。结果表明,Chemerin与ChemR23基因共同表达于藏鸡所有被检测的组织中,且Chemerin主要在肝、脂肪、肺和肾等组织中表达,而ChemR23在脂肪组织中的表达量最高(P<0.01)。Chemerin与ChemR23在不同发育阶段胸肌和脂肪组织中的表达无性别差异,均在孵化0日龄的胸肌中表达水平最高,在154日龄的皮下脂肪组织中表达水平最高。Chemerin与ChemR23基因在腿肌中的表达存在性别差异,Chemerin与ChemR23在119日龄母鸡腿肌中的表达水平极显著高于其他几个时期(P<0.01);但Chemerin在0和210日龄公鸡腿肌中的表达水平极显著高于其他几个时期(P<0.01),ChemR23在210日龄公鸡腿肌表达水平最高(P<0.01)。Chemerin与ChemR23mRNA表达量与胸肌IMF含量呈正相关,Chemerin基因mRNA表达水平与腿肌IMF含量呈负相关,ChemR23基因mRNA表达水平与公鸡腿肌IMF含量呈负相关,与母鸡腿肌IMF含量呈正相关。研究结果提示,Chermerin及其受体ChemR23基因可能在藏鸡生长发育过程中对肌内脂肪沉积发挥重要作用,本研究结果为进一步阐明Chermerin及其受体ChemR23基因在藏鸡脂肪代谢中的生物学功能奠定基础。

This study aimed to clarify the tissue and sequential expression profiles of Chemerinand ChemR23 genes in Tibetan chicken,and to investigate the impacts of the 2 genes expressions on intramuscular fat(IMF)deposition.In this study,Tibetan chicken(0-210day)was used as experimental animals and the RT-PCR method were employed to amplify the Chemerin and ChemR23 genes.Their relative expression was measured by fluorescent quantitative PCR in various tissues and at different developmental stages.The correlation analysis was performed between gene expression and IMF content.The results indicated that expression of Chemerin and ChemR23 was detected broadly in different tissues.Chemerin was mostly expressed in liver,adipose tissue,lung and kidney of Tibetan chicken,while ChemR23 was expressed in adipose tissue at significantlyhigher levels than in other tissues(P<0.01).The highest levels were in breast muscle at day 0and in adipose tissue at day 154,but the expression patterns of the 2genes in breast muscle and adipose tissue at different developmental stages were not correlated with gender.However,their expression patterns in the thigh muscle were correlated with gender.In female thigh muscle,the relative expression levels of both genes were significantly higher at day 119 than other periods(P<0.01),but in male thigh muscle,the relative expression level of Chemeringenes were significantly higher at day 0and 210 than other periods(P<0.01).The peak expression of ChemR23 presented at day 210 in male thigh muscle.The expression of Chemerinand ChemR23 mRNA was positively correlated with IMF content in breast muscle,whereas Chemerinexpression was negatively correlated with IMF content in thigh muscle.Interestingly,ChemR23 expression was negatively and positively correlated with IMF content of male and female thigh muscle,respectively.The results suggest that Chemerinand ChemR23 genes may play critical roles in IMF deposition during growth and development of Tibetan chicken,which will help us to understand the biological functions of Chemerinand ChemR23 genes in fat metabolism of Tibetan chicken.