转录因子USF1调控鸡小肠上皮细胞中糖类转运蛋白表达

Transcription Factor USF1 Regulate the Expression of Monosaccharide Transporters in Chicken Intestinal Epithelial Cells

为了研究转录因子USF1在鸡小肠上皮细胞中调控糖转运蛋白基因表达的机制,本试验在生物信息学分析的基础上通过构建真核表达载体pcDNA3.1-USF1,在鸡小肠上皮细胞中过表达转录因子USF1,用绝对荧光定量PCR的方法检测分析了USF1对SGLT1、GLUT2和GLUT5基因表达的影响。结果表明,受到USF1基因过表达的影响,糖类转运蛋白GLUT2和SGLT1 mRNA在鸡小肠上皮细胞中的表达量极显著增高(P<0.01);而GLUT5mRNA表达量没有显著变化(P>0.05)。结合本研究结果,经过对这3个糖类转运蛋白基因5′上游调控区USF1结合位点的深入分析,认为在鸡小肠上皮细胞中转录因子USF1对糖转运蛋白基因GLUT2和SGLT1的表达具有正向调控的作用。

In order to study the potential regulation mechanism of the USF1 on monosaccharide transporters in chicken intestinal epithelial cells,we built the eukaryote expression vector pcDNA3.1-USF1 on the basis of bioinformatics analysis and made the transcription factor USF1 overexpressed in chicken intestinal epithelial cells.Besides,we detected and analyzed the influence of USF1 on the mRNA expressions of SGLT1,GLUT2 and GLUT5by Real-time PCR.The results indicated that the over-expression of USF1 gene significantly up-regulated the mRNA expressions of GLUT2 and SGLT1in chicken intestinal epithelial cells(P<0.01),while the expression of GLUT5 were not changed significantly(P>0.05).Combining the results with the bioinformatics analysis of the USF1 binding sites on the 5′up-stream regulation area of the 3 monosaccharide transporters,our results indicated that the transcription factor USF1 positively regulated the expression of SGLT1 and GLUT2in chicken intestinal epithelial cells.