黄籽甘蓝型油菜核心种质的SSR标记分析

Genetic Diversity Analysis of Core Collection of Yellow-Seeded Brassica napus Using SSR Markers

核心种质筛选是植物遗传资源研究和利用的便捷途径,利于种质资源杂种优势利用.遗传多样性分析有利于最优亲本组合的鉴定,以期能够产生遗传变异最大的后代群体和促进不同资源的有利基因渗透到栽培品种.该研究通过表型数据分析表明,初选核心种质能够有效地代表参试群体的遗传多样性,利用20对SSR标记对20份黄籽甘蓝型油菜的核心种质进行了遗传多样性分析,共检测出128个等位基因,每对引物在不同材料之间的等位基因数在4~11之间,平均位点为6.40个,多态信息量PIC值在0.81~0.92之间,通过UPGMA法,核心种质的遗传相似系数介于0.17~0.61之间,说明黄籽甘蓝型油菜核心种质具有较丰富的遗传多样性.该研究为甘蓝型黄籽油菜基因资源的挖掘和黄籽杂交育种的利用提供了理论基础.

Constructing core collections is a convenient way to study and utilize plant germplasm resources , which could be used in crop heterosis in hybrid .Assessment of genetic diversity benefits the identification of optimal parental combinations to produce segregating offspring with maximum genetic variability ,and facilitates the introgression of favorable genes from various germplasm into commercial cultivars .In this research ,core collection could reflect the genetic diversity of entire collection based on the phenotypic traits in yellow‐seeded lines of B? napus .The results showed that 20 SSR primer combinations were em‐ployed to evaluate the genetic diversity of 20 core collection of yellow‐seeded lines rapeseed ,and a total of 128 alleles were detected in 20 core collection .At each SSR locus ,4 to 11 alleles could be detected ,with average of 6?40 and polymorphism information content (PIC) 0?81 to 0?92 .The genetic similarity among 20 core collection was calculated ,with varied 0?17 to 0?61 ,using the UPGMA cluster analysis .The grea‐ter genetic diversity of 20 core collection of yellow‐seeded lines was fully revealed in this study ,which pro‐vided the theoretical foundations for further exploring ,hybridization and breeding of the yellow‐seeded rapeseed germplasm in the future .