不同工艺制备的白术多糖对小鼠血清中细胞因子的影响

Influence of AtractylodesmacrocephalaPolysaccharides Produced by Different Preparation Technologies on Cytokines of Mouse Serum

研究白术在不同制备工艺条件下得到的白术多糖(PAM)对小鼠血清中细胞因子的影响,采用传统水提和超声法提取白术多糖(PAM),以苯酚硫酸比色法测定多糖含量.通过腹腔注射环磷酰胺,建立免疫抑制模型,将提取的PAM连续灌胃小白鼠14d,观察PAM对小白鼠血清中IL-2,IL-4和IFN-γ含量的影响.结果发现,两种方法提取的PAM对小鼠日增质量和免疫器官指数的影响不明显,但均能极显著提高免疫低下小鼠血清中IL-4的含量.结果表明,传统水提和超声法提取的PAM均能在不影响生长性能的基础上,促进小鼠血清中细胞因子IL-4的表达,缓解免疫应激,提高机体免疫功能,但水提法优于超声提取法.

To study the influence of A tractylodes macrocephala polysaccharides produced by different preparation technologies on cytokines of mouse serum .PAM extracted by the traditional water extraction and ultrasonic extraction ,which’s content were detected by the phenol sulfuric acid colorimetric .Immuno‐compromised mouse model were prepared by intraperitoneal injection of cyclophosphamide ,the influence of PAM on IL‐2 ,IL‐4 and IFN‐γ in mouse serum was observed by administering orally in the following 14 days .It was found that the ADG and Organ index of each experimental group mice were similar to the con‐trol and model group ,but can significantly improve the immunocompromised mouse serum IL‐4 levels . The results show that ,PAM by traditional water extraction and ultrasonic extraction can promote mouse serum cytokines IL‐4 expression ,alleviate immune stress ,and improve immune function ,water extraction is better than ultrasonic extraction .