重庆市10大名柚果实酚类物质质量分数及抗氧化活性研究

Phenolic Contents of Ten Famous Chongqing Pummelo(Citrus grandis)Cultivars and Their Antioxidant Capacities

该研究测定了重庆市10大名柚成熟果实囊衣、汁胞、种子3个部位的总酚、总黄酮质量分数,同时采用1,1-二苯基-2-三硝基苯肼(DPPH)、铁离子还原能力测定(FRAP)、2,2-朕氮-二(3-乙基-苯并噻唑-6-硫酸)二铵盐(ABTS)共3种方法对其抗氧化活性进行了评价,并进一步分析了不同部位的酚类物质质量分数与其抗氧化活性的相关性.结果表明:总酚、总黄酮质量分数最高的部位分别是汁胞和种子.囊衣、汁胞、种子三3个部位总酚质量分数最高的分别是琯溪蜜柚、真龙柚3号和真龙柚3号;总黄酮质量分数最高的分别是琯溪蜜柚、梁平柚78-8和渝北沙田柚.其次,该研究还发现,抗氧化活性与检测方法有关,用DPPH,FRAP,ABTS共3种方法测得的抗氧化活性最强的部位分别是汁胞、汁胞和囊衣.综合抗氧化能力指数表明,琯溪蜜柚囊衣、梁平柚78-8汁胞、渝北沙田柚种子的抗氧化活性最强.抗氧化活性与总酚、总黄酮质量分数的相关性分析结果表明,囊衣总酚、总黄酮质量分数与ABTS的相关性极具有统计学意义(p<0.01);汁胞总酚、总黄酮质量分数与DPPH,FRAP,ABTS的相关性具有统计学意义(p<0.05);种子总酚质量分数与DPPH,FRAP,ABTS,总黄酮质量分数与FRAP的相关性具有统计学意义(p<0.05).

The total phenolic compound and total flavonoid contents in the SM (segment membrane) ,JS (juice sac) and seeds of ten famous Chongqing mature pummelo (C. grandis) cultivars were determined in the present study .In addition ,the antioxidant capacities of the three parts were evaluated by DPPH , FRAP and ABTS methods ,and the correlations between the contents of phenolics and their antioxidant ca-pacities in each part were analyzed .JS and seeds were shown to have the highest total phenolic compound and total flavonoid contents .C. maxima (Burm.) Merr. cv. Guanxi Yu ,C. maxima (Burm.) Merr. cv.Zhenlong Yu No.3 and C. maxima (Burm.) Merr. cv. ZhenlongYu No.3 were found to have the highest total phenolic compound contents in their SM ,JS and seeds ,respectively .C. maxima (Burm.) Merr. cv.Guanxi You ,C. maxima (Burm.) Merr. cv. Liangpin You 78-8 and C. maxima (Burm.) Merr. cv.Shatian You from Yubei contained the highest total flavonoids .In addition ,antioxidant capacities varied significantly depending on the test methods .The parts having highest antioxidant capacities were JS ,JS and SM evaluated with DPPH ,FRAP and ABTS ,respectively .the SM of C. maxima (Burm.) Merr. cv.Guanxi You ,the JS of C. maxima (Burm.) Merr. cv. Liangpin You 78-8 and the seeds of C. maxima (Burm.) Merr. cv. Shatian You from Yubei exhibited the highest antioxidant capacities .Correlation anal-ysis indicated that the total phenolic compound and the total flavonoid contents of SM were in highly sig-nificant positive correlations with the ABTS method (p<0.01) ,and significant positive correlations were observed between the total phenolic compound and the total flavonoid contents of JS and the DPPH , FRAP ,ABTS test methods (p<0.05) .Total phenolic compound content of seeds was in significant posi-tive correlations with the DPPH ,FRAP ,ABTS test methods and total flavonoid content of seeds was in significant positive correlation with the FRAP method (p<0.05) .