外源SNP,Spd对盐碱胁迫下射干幼苗体内抗氧化酶活性的影响

Effects of Exogenous SNP and Spd on the Activity of Antioxidant Enzyme System in Belamacanda chinensis(L.)DC.Seedlings Under Saline-Alkali Stress

为了模拟自然环境下的盐碱地环境,使用混合盐碱(NaCl∶Na2SO3∶NaHCO3∶Na2CO3为9∶1∶9∶1)处理射干幼苗,之后引入硝普钠(SNP)和亚精胺(Spd)外源,研究了整个过程中射干幼苗体内超氧化物歧化酶(SOD),过氧化物酶(POD)和过氧化氢酶(CAT)的活性变化.结果表明:在盐碱胁迫下,射干幼苗体内的SOD,POD,CAT活性随着处理时间的延长而增加,施加外源SNP和Spd处理后,活性继续上升.实验证明:耐盐性植物射干在盐碱胁迫下具有自修复功能,能提高体内抗氧化酶系活性,外源SNP与Spd可进一步增加植物体内的抗氧化酶系活性,从而清除盐碱胁迫下活性氧对植物体的损伤.

In order to simulate the natural salt‐alkali soil condition ,a mixture of salt and alkali (NaCl∶Na2 SO3 ∶NaHCO3 ∶Na2 CO3 =9∶1∶9∶1) was used to treat Belamacanda chinensis (L. ) DC .seedlings . Then ,SNP and Spd were introduced as extraneous sources ,and the variation of SOD ,POD and CAT con‐tents in them was studied .The result showed that the contents of SOD ,POD and CAT increased with pro‐cessing time under salt‐alkali stress ,and that after the introduction of exogenous SNP and Spd ,the con‐tent level of the above three substances continued to increase .T he above results indicated that the salt‐tol‐erant plant B .chinensis (L. ) DC had a self‐healing ability under salt‐alkali stress by increasing the con‐tents of antioxidant enzymes ,and that exogenous SNP and Spd could further increase the activities of the antioxidant enzymes ,which would remove the harmful active oxygen within plants incurred by salt‐alkali stress .